白藜芦醇通过调节Ca2+浓度对小鼠外周疼痛的影响及作用机制

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摘要目的：探讨Ca2+通道，特别是L型钙通道和钙/咖啡因敏感池，是否参与白藜芦醇的镇痛作用。方法：小鼠单独使用反式白藜芦醇（10、20、40 mg/kg，p.o.）30 min后，进行甩尾实验，记录痛阈值，评价对痛觉的反应；白藜芦醇（10、20 mg/kg，p.o.）联合使用MK801（0.5 mg/kg，i.p.）或尼莫地平（2.5、5、10 mg/kg，i.p.）或CaCl2（25、50、100、200 nmol/只，i.c.v.）或兰尼定（0.25、0.5、1、2 nmol/只，i.c.v.）或EGTA（5、15、30 nmol/只，i.c.v.）30 min后，同样进行甩尾实验，记录痛阈值，评价对痛觉的反应。结果：甩尾实验中，白藜芦醇增加小鼠的甩尾潜伏期（P＜0.01）。联合应用MK801或尼莫地平增强了白藜芦醇的镇痛作用（P＜0.05），中枢给予Ca2+后，降低了白藜芦醇的镇痛作用（P＜0.05）；而联合使用EGTA或兰尼定增强了白藜芦醇的镇痛作用（P＜0.05）。结论：当白藜芦醇联合使用MK801或尼莫地平或EGTA或兰尼定时，白藜芦醇产生的镇痛作用明显增强；而白藜芦醇联合使用Ca2+后，白藜芦醇产生的镇痛作用明显降低。白藜芦醇可能是通过Ca2+通道和钙库/咖啡因敏感池发挥镇痛作用。

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	王林
	潘建春

关键词 ：白藜芦醇, 钙通道, 疼痛, 小鼠

Abstract：Objective: To explore whether Ca2+, especially L-voltage-gated Ca2+ channels and Ca2+/caffeine-sensitive pools are involved in a algesic, effects and mechanisms in resveratrol-induced antinociception. Methods: Tail flick test was used to assess allodynia in mice treated with resveratrol (10, 20, 40 mg/kg, p.o.) alone or the combination of resveratrol or MK801 (0.5 mg/kg, i.p.) or nimodipine (2.5, 5, 10 mg/kg, i.p.) or CaCl2 (25, 50, 100, 200 nmol per mouse, i.c.v.) or ryanodine (0.25, 0.5, 1, 2 nmol per mouse, i.c.v.) or EGTA (5, 15, 30 nmol per mouse, i.c.v.). Results: The results showed that resveratrol increased the tail flick latency in the tail flick test (P<0.01). The effect of resveratrol was strengthened by MK801 or nimodipine (P<0.05). Central administration of Ca2+, however, diminished the antinociceptive effect of resveratrol (P<0.05). In contrast EGTA or ryanodine administered centrally improved the antinociceptve effect (P<0.05). Conclusion: The effect of resveratrol was enhanced by MK801, nimodipine, EGTA and ryanodine, while central administration of Ca2+, reduced the antinociceptive effects of resveratrol. The findings suggest that resveratrol produces effects on antinociception through regulation of calcium channels and calcium/caffeine-sensitive pools.

Key words： resveratrol calcium channels pain mice

PACS:

通讯作者: 潘建春，教授，Email：wenzhoupan2003@163.com

作者简介: 王林（1986-），男，江苏涟水人，药师，硕士

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I1/53

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