小鼠心脏缝隙连接蛋白节律性表达与生物钟系统相关性

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摘要目的：观察小鼠心脏内缝隙连接蛋白基因Cx40和Cx43的表达是否有节律性，及其与生物钟系统的相关性。方法：经过2周的单独饲养，每6 h采集野生型和clock变异小鼠的心房、心室组织，用荧光定量PCR法观察生物钟基因Per2、Dbp和缝隙连接蛋白基因Cx40、Cx43的表达水平。结果：在野生型小鼠的心脏中，生物钟基因Per2、Dbp及缝隙连接蛋白基因Cx40、Cx43的表达有显著的节律性；在clock变异小鼠心脏中，Per2、Dbp表达节律消失，而Cx40和Cx43表达虽然保持节律性，但其节律的位相发生显著改变，在心房中显著前移而在心室中显著后移。在心室中，Cx40、Cx43表达水平也增高。结论：缝隙连接蛋白基因Cx40和Cx43在心脏中呈节律性表达，这种节律性可能受到生物钟系统的调节。

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	柴莹
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关键词 ：缝隙连接, Clock变异, 昼夜节律, 小鼠

Abstract：Objective: To examine the circadian expression of cardiac connexin genes in the mouse and its correlation with biological clock. Methods: After 2-week constant housing, the atrium and ventricle of wild and Clock mutant mice were sampled every 6 hours. The expressions of Clock genes (Per2 and Dbp), cardiac connexin genes (Cx40 and Cx43) were determined by the real-time quantitative PCR. Results: The mRNA levels of all examined genes showed substantial circadian rhythms in wild type mice. In clock mutant mice, Per2 and Dbp lost circadian rhythms in both atrium and ventricle, while Cx40 and Cx43 still had circadian rhythms but phase-shifted significantly, which were phase-advanced in atrium and phase-delayed in ventricle. The Cx40, Cx43 expressions were upregulated in ventricle. Conclusion: Cardiac Cx40 and Cx43 gene expressions shows clear circadian rhythm in mice and may be associated with the biological clock system.

Key words： connexin Clock mutant circadian rhythm mice

PACS:

基金资助:宁波市自然科学基金资助项目（2014610269）；浙江省医药卫生一般研究计划项目（2016KYB262）

通讯作者: 童茂清，副教授，Email：ganbarusy@163.com

作者简介: 柴莹（1982-），女，浙江宁波人，主治医师

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2018/V48/I1/42

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