Comprehensive mapping of immunodominant B-cell epitopes and T-cell epitopes enriched areas of sperm associated antigen 9
Mao Chenchen1, Ye Sisi2, Zhang Teming1, Han Zheng1, Xue Xiangyang2, Shen Xian1,3
1.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Basic Medical College of Wenzhou Medical University, Wenzhou, 325035; 3.Department of Gastrointestinal Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
Mao Chenchen,Ye Sisi,Zhang Teming, et al. Comprehensive mapping of immunodominant B-cell epitopes and T-cell epitopes enriched areas of sperm associated antigen 9[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2018, 48(1): 1-6.
Abstract：Objective: To predict the B-cell epitopes and T-cell epitopes enriched areas of sperm associated antigen 9 (SPAG-9). Methods: On account of the full-length sequence of SPAG-9, using bioinformatics software including the hydrophilic plot technique, polarity parameters, surface probability, antigenic index, and the secondary structure, along with its flexible regions and transmembrane region analysis, the B-cell epitopes of the SPAG-9 protein were predicted. Antigenic index further calculated to determine the target epitopes. Meanwhile, SYFPEITHI, NetCTL and IEDB were used to predict HLA-A2 and HLA-A24 restricted CTL epitopes. Finally, B cell epitopes and CTL epitopes were combined to predict the SPAG-9 epitope enriched areas. Results: The predicted B-cell epitopes of the SPAG-9 might exist in N-terminal of amino acid sequence: 197-201, 236-243, 249-253, 291-299, 306-312, 324-327, 332-337, 348-352, 488-494, 539-542, 546-550, 565-573, 699-702, 802-807, 869-873, 875-880, 882-887, 1173-1178, 1195-1200. The HLA-A2 restricted CTL epitopes which scored high of all the three methods were peptides about 19-27, 49-57, 50-58, 56-64, 343-351, 381-389, 413-421, 447-455, 521-529, 551-559, 665-673, 733-741, 837-845, 926-934, 950-958, 964-972, 998-1006, 1016-1024, 1094-1102, 1140-1148, 1150-1158 and the HLA-A24 restricted CTL epitopes were peptides about 31-39, 137-145, 517-525, 943-951. Combine the above results, the peptides about 332-352 (SAENEEKSEVQAIIESTPELD), 551-573 (SIWQFFSRL FSSSSNTTKKPEPP) might be the epitopes enriched areas of SPAG-9. Conclusion: Bioinformatics prediction shows that the peptides about 332-352 and 551-573 might be the epitopes enriched areas of SPAG-9.
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