肥胖对雌性大鼠交感肾上腺系统的长期程序化作用

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摘要目的：研究肥胖对雌性大鼠交感肾上腺系统的长期程序化作用。方法：将雌性SD大鼠在出生当日分成2组：①实验组，小窝喂养，每窝4只；②对照组，正常喂养，每窝12只。每周测体质量，出生后28 d开始监测阴道口初开放时间和阴道细胞学涂片。出生后10周，用免疫组织化学方法测定肾上腺和卵巢交感神经兴奋性的改变；用原位杂交技术测定中枢蓝斑核的酪氨酸羟化酶（TH）mRNA的表达变化。结果：与对照组相比，实验组动物明显肥胖，持续终生；青春期启动明显提早[实验组（35.5±0.8）d，对照组（37.6±0.3）d]；卵巢和肾上腺的TH免疫染色明显增强和蓝斑核的TH mRNA表达明显增加，其差异均有统计学意义（均P＜0.05）。结论：肥胖通过上调交感肾上腺兴奋性影响雌鼠的生殖功能。

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	吴雪清1
	杨思蒙1
	李月华1
	周强勇1
	陈海燕1
	李海鹰2

关键词 ：肥胖, 肾上腺, 卵巢, 生殖功能, 交感神经, 大鼠

Abstract：Objective: To investigate the long-term programming effects of obesity on the sympathoadrenal system in female rats. Methods: The litter size was adjusted to 4 pups per litter for the treatment group and 12 neonates per dam in the controls at postnatal day 0. Body weights were measured weekly. Vaginal opening and vaginal cytology smears were monitored from postnatal day 28. At the 10 weeks of age, the sympathetic nerve activity in adrenals and ovaries were detected by immunohistochemistry and the expression of TH mRNA in the locus coeruleus were investigated by in situ hybridization. Results: As compared to normal litters, the small litter rats were obesity throughout life. The puberty onsets were obviously advanced (35.5±0.8 d in treatment groups vs.37.6±0.3 d) in controls. The immuno-staining of TH were significantly enhanced in both the ovaries and adrenals in small litters. The level of TH mRNA in the locus coeruleus was significantly increased in small litters. All the differences were statistically significant (P<0.05). Conclusion: Obesity programmed the reproductive function of female rats by up-regulation of the sympathoadrenal system.

Key words： obesity adrenal ovary reproductive function sympathetic nerve rats

收稿日期: 2013-11-25

基金资助:国家自然科学基金资助项目（81100392）；浙江省自然科学基金资助项目（Y2110606）；教育部留学回国人员科研启动基金资助项目（2012-1707）

通讯作者: 李海鹰，主任医师，Email：lihaiying111@gmail.com。

作者简介: 吴雪清（1971-），女，浙江温州人，教授，博士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2014/V44/I3/157

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