慢性低O2高CO2对小鼠海马细胞凋亡、caspase-3和p38 MAPK活性的影响

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摘要目的：研究慢性低O2高CO2对小鼠海马细胞凋亡、caspase-3以及p38 MAPK活性的影响。方法：雄性C57BL/6小鼠24只，随机分成2组：4HH组（低O2高CO2组12只）和NC组（正常对照组12只）。4HH组饲养于氧舱中，舱内O2浓度为9%～11%，CO2浓度为5%～6%，每天8 h，每周6 d，共4周，其余时间与NC组一样，生活于正常环境。原位末端标记（TUNEL）法检测海马细胞凋亡指数（AI），caspase-3活性检测试剂盒测定海马caspase-3活性，Western blot检测海马p-p38 MAPK蛋白的表达。结果：4HH组小鼠海马细胞凋亡指数（AI）、caspase-3活性及p-p38 MAPK蛋白含量显著高于NC组（P＜0.01）。结论：慢性低O2高CO2促进小鼠海马细胞凋亡，促进caspase-3以及p38 MAPK活化。

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关键词 ：低氧, 高碳酸血症, p38丝裂原活化蛋白激酶类, caspase-3, 凋亡, 小鼠, 海马

Abstract：Objective: To investigate the effect of the chronic hypoxic-hypercapnia in apoptosis and activity of p38 MAPK and caspase-3 of hippocampus in mouse. Methods: Twenty-four male mice were randomly divided into two groups: the hypoxic-hypercapnia 4 weeks (4HH) group and normal control (NC) group. The 4HH groups in the special cantainer were exposed to an atmosphere containing 9%～11% O2 and 5%～6% CO2 8 hours a day, 6 days a week for 4 weeks. The two groups lived in the same room in the rest time. Apoptosis index (AI) in hippocampus was counted by TUNEL staining. Caspase-3 activity was measured by a caspase-3 activity assay kit. p-p38 MAPK protein in hippocampus was measured by Western blot. Results: Compared with NC group the AI, caspase-3 activity, and the p-p38 MAPK protein expression in hippocampus were significantly increased in 4HH group (P<0.01). Conclusion: The chronic hypoxic-hypercapnia promotes apoptosis and the activation of caspase-3 and p38 MAPK of hippocampus in mouse.

Key words： hypoxia, hypercapnia p38 mitogen-activated protein kinases caspase-3 apoptosis mouse hippocampus

收稿日期: 2017-03-30

通讯作者: 王小同，主任医师，Email：wangxt22@163.com。

作者简介: 任惠明（1984-），男，山西吕梁人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I12/895

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