冬凌草甲素对人脐静脉内皮细胞的抑制作用

摘要
图/表
参考文献
相关文章 (4)

全文: PDF (2901 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的：研究冬凌草甲素对人脐静脉内皮细胞增殖、凋亡、迁移、侵袭和成管的影响。方法：培养人脐静脉内皮细胞（HUVEC），分别选择噻唑蓝比色法（MTT）、流式细胞荧光分选法（FACS）、穿透小室法、管腔形成实验法检测HUVEC的增殖、凋亡、迁移、侵袭和成管。采用相对荧光定量PCR检测VEGFA和VEGFR2基因的表达量。结果：冬凌草甲素可诱导HUVEC凋亡，抑制HUVEC增殖、迁移、侵袭和成管（P＜0.05）。冬凌草甲素可降低VEGFA和VEGFR2基因的表达量（P＜0.05）。结论：冬凌草甲素可能通过降低VEGFA和VEGFR2基因的表达量，进而对HUVEC产生良好的抑制作用。

	服务
	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	田丽莉
	朱国福
	盛东来

关键词 ：冬凌草甲素, 人脐静脉内皮细胞, 血管内皮生长因子A, 血管内皮生长因子受体2

Abstract：Objective: To study the inhibition effect of oridonin on human umbilical vein endothelial cells. Methods: The research chose human umbilical vein endothelial cells (HUVEC): cell viability was assessed by MTT assay, cell apoptosis was assessed by FACS analysis, cell migration and invasion was assessed by trans well chambers and image analysis, and cell angiogenesis was also investigated using tube formation assay. The expression of VEGFA and VEGFR2 were measured by real-time fluorescent quantitative PCR. Results: Oridonin induced HUVEC apoptosis and inhibited the proliferation, migration, invasion, and tube formation (P<0.05). The expression of VEGFA and VEGFR2 were down-regulated (P<0.05). Conclusion: Oridonin can inhibit HUVEC, which maybe connected with VEGFA and VEGFR2 down-regulation expression.

Key words： oridonin human umbilical vein endothelial cells vascular endothelial growth factor A vascular endothelial growth factor receptor-2

收稿日期: 2017-01-05

基金资助:国家自然科学基金青年基金资助项目（31300908）

通讯作者: 朱国福，教授，博士生导师，Email：zgfzyy2015@sina.com

作者简介: 田丽莉（1982-），女，河南焦作人，中药师，博士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I12/880

[1] 冯耀荣,陈红淑. 冬凌草甲素抗肿瘤活性研究进展[J]. 中国中医药科技, 2016, 23(1): 125-126.
[2] 田丽莉, 盛东来, 朱国福. 冬凌草甲素抑制血管生成活性及作用机制[J]. 中国实验方剂学杂志, 2016, 22(5): 166-171.
[3] 魏仙, 姚于勤, 杨金亮. 新生血管生成模型及应用[J]. 生物技术通讯, 2015, 26(3): 421-425.
[4] BOUïS D, HOSPERS G A, MEIJER C, et al. Endothelium in vitro: a review of human vascular endothelial cell lines for blood vessel-related research[J]. Angiogenesis, 2001, 4 (2): 91-102.
[5] SHIBUYA M. Vascular endothelial growth factor (VEGF) and its receptor (VEGFR) signaling in angiogenesis: A crucial target for anti-and pro-angiogenic therapies[J]. Genes Cancer, 2011, 2(12): 1097-1105.
[6] HOLMES D I, ZACHARY I. The vascular endothelial growth factor (VEGF) family: angiogenic factors in health and disease[J]. Genome Biol, 2005, 6(2): 209.
[7] OLSSON A K, DIMBERG A, KREUGER J, et al. VEGF receptor signalling-in control of vascular function[J]. Nat Rev Mol Cell Biol, 2006, 7(5): 359-371.
[8] SHIBUYA M. Differential roles of vascular endothelial growth factor receptor-1 and receptor-2 in angiogenesis[J]. J Biochem Mol Biol, 2006, 39(5): 469-478.
[9] MEADE-TOLLIN L C, WIJERATNE E M, COOPER D, et al. Ponicidin and oridonin are responsible for the antiangiogenic activity of Rabdosia rubescens, a constituent of the herbal supplement PC SPES[J]. J Nat Prod, 2004, 67(1): 2-4.
[10] DONG Y, ZHANG T, LI J, et al. Oridonin inhibits tumor growth and metastasis through anti-angiogenesis by blocking the Notch signaling[J]. PLoS One, 2014, 9(12): e113830.
[11] 孙晓佳, 张月英. 血管生成主要信号通路[J]. 国际肿瘤学杂志, 2010, 37(2): 95-97.
[12] LI Y L, ZHAO H, REN X B. Relationship of VEGF/VEGFR with immune and cancer cells: staggering or forward?[J]. Cancer Biol Med, 2016, 13(2): 206-214.
[13] KUMAR M, DHATWALIA S K, DHAWAN D K. Role of angiogenic factors of herbal origin in regulation of molecular pathways that control tumor angiogenesis[J]. Tumour Biol, 2016, 37(11): 14341-14354.
[14] LU K, BHAT M, BASU S. Plants and their active compounds: natural molecules to target angiogenesis[J]. Angiogenesis, 2016, 19(3): 287-295.