树脂改性玻璃离子Vitrebond的光固化时间对细胞毒性的影响

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摘要目的：探讨树脂改性玻璃离子Vitrebond不同光固化时间下对细胞毒性的影响，比较琼脂扩散法和MTT法的细胞毒性敏感度。方法：Vitrebond根据说明书制成2 mm厚样本，在强度1 000 mw/cm2光照下，分成4组，经不同光照时间（0、15、30、45 s）后作为实验组，苯酚为阳性对照组，MEM、棉花籽油为阴性对照组。每组各含样本8个，在24和48 h后，应用琼脂扩散法，分别评估L929细胞脱色区和L929细胞溶解指数；实验第5天，通过MTT法，比较各组中Vitrebond对L929细胞的细胞毒性。结果：琼脂扩散法中，阳性对照及未光固化组呈现严重细胞毒性，阴性对照及各光固化组无细胞毒性。MTT法中，实验组均有较强细胞毒性，且组间差异无统计学意义（F=1.452，P＞0.05）。结论：1 000 mw/cm2光照下，Vitrebond的光固化时间对细胞毒性无影响；琼脂扩散法敏感度不如MTT法。

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	杜若茜
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	麻健丰

关键词 ：树脂改性玻璃离子, 细胞毒性, 光固化, Vitrebond, 琼脂扩散法, MTT法

Abstract：Objective: To investigate the influence of curing time of curing light on the cytotoxicity of Vitrebond, and compare the cytotoxic sensitivity of Agar diffusion test and MTT assay. Methods: Vitrebond of 2 mm thickness were light-cured for 15, 30, 45 seconds with 1 000 mw/cm2 curing light. The effects of different curing time of Vitrebond on L929 cell were evaluated by Agar diffusion test and MTT assay after 24 h and 48 h. Results: The evaluation of agar diffusion found the positive control and the specimen without any curing induced severe cytotoxity, there was no cytotoxicity in negative control, any of specimens and any of MEM or cottonseed oil extracts. In MTT test, Vitrebond showed a great cytotoxicity whatever the curing time was. There was no statistical difference of cytotoxicity cured for 15, 30, and 45 seconds (F=1.452, P>0.05). Conclusion: There are no difference of cytotoxicity of Vitrebond cured with 1 000 mw/cm2, with different curing time. Agar diffusion test is lower sensitive than MTT test to detect the cytotoxicity.

Key words： resin-modified glass-ionomer cements cytotoxicity light curing vitrebond agar diffusion MTT

收稿日期: 2017-05-11

基金资助:温州市科技计划项目（Y20140335）。

通讯作者: 麻健丰，教授，硕士生导师，Email：dentistmacn@aliyun.com。

作者简介: 杜若茜（1984-），女，浙江永嘉人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I12/875

[1] MUSA A, PEARSON G L, GELBIER M. In vitro investigation of fluorideion release from four resin-modified glass polyalkenoate cements[J]. Biomaterials, 1996, 17(10):1019-1023.
[2] LOVELL L G, NEWMAN S M, DONALDSON M M, et al. The effect of light intensity on double bond conversion and flexural strength of a model, unfilled dental resin[J]. Dent Mater, 2003, 19(6): 458-465.
[3] SPAHL W, BUDZIKIEWICZ H, GEURTSEN W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography mass spec-trometry[J]. J Dent, 1998, 26(2): 137-145.
[4] ASMUSSEN E. Restorative resins: hardness and strength vs. quantity of remaining double bonds[J]. Scand J Dent Res, 1982, 90(6): 484-489.
[5] MITRA S B, LEE C Y, BUI H T, et al. Long-term adhesion and mechanism of bonding of a paste-liquid resin-modified glass-ionomer[J]. Dent Mater, 2009, 25(4): 459-466.
[6] 郭晓伟, 严敏, 宫海环, 等. 复合树脂细胞毒性检测方法的进展[J]. 口腔医学, 2016, 9(36): 845-848
[7] PALMER G, ANSTICE H M, PEARSON G J. The effect of curing regime on the release of hydroxyethyl methacrylate (HEMA) from resin-modified glass-ionomer cements[J]. J Dent, 1999, 27(4): 303-311.
[8] STANISLAWSKI L, DANIAU X, LAUTI A, et al. Factors responsible for pulp cell cytotoxicity induced by resin-modified glass ionomer cements[J]. J Biomed Mater Res, 1999, 48(3): 277-288.
[9] DE SOUZA COSTA C A, HEBLING J, HANKS C T. Effects of light-curing time on the cytotoxicity of a restorative resin composite applied to an immortalized odontoblast-cell line[J]. Oper Dent, 2003, 28(4): 365-370.
[10] ARANHA A M,GIRO E M,SOUZA P P,et al. Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line[J]. Dent Mater, 2006, 22(9): 864-869.
[11] ERGUN G, EGILMEZ F, YILMAZ S. Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led[J]. J Appl Oral Sci, 2011, 19(3): 286-292.
[12] TRUMPAITE-VANAGIENE R, BUKELSKIENE V, ALEKSEJUNIENE J, et al.Cytotoxicity of commonly used luting cements—An in vitro study[J]. Dent Mater J, 2015, 34(3): 294-301.
[13] SALEHI S, GWINNER F, MITCHELL J C, et al. Cytotoxicity of resin composites containing bioactive glass fillers[J]. Dent Mater, 2015, 31(2): 195-203.
[14] MOSMANN T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays[J]. J Immunol Methods, 1983, 65(1-2): 55-63.
[15] YY/T 0127.9-2001口腔材料生物学评价, 第2单元: 口腔材料生物试验方法, 细胞毒性试验: 琼脂覆盖法及分子滤过法[S].