Preparation vitamin B2 modified multiwall carbon nanotubes electrode and determination of DNA by electrochemistry method
ZHANG Tiantian1, YANG Lizhu2.
1.Department Of Chinese Traditional Medicine, the First Affiliated Hospital of Zhejiang Chinese Medicine University, Hangzhou, 310000; 2.School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035
ZHANG Tiantian,YANG Lizhu.. Preparation vitamin B2 modified multiwall carbon nanotubes electrode and determination of DNA by electrochemistry method[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(12): 869-874.
Abstract：Objective: The research on interaction between vitamin B2 which was on the vitamin B2/multiwall carbon nanotube modified electrode and DNA by electrochemistry and UV spectra methods was performed. Methods: There was an obvious pair of reduction and oxidation peaks on the prepared vitamin B2/multiwall carbon nanotube modified electrode. Both reduction and oxidation peak currents of vitamin B2 decreased with no change in the peak potentials after adding DNA. Through the test of the electrochemical parameters in the absence and presence of DNA, it was found that after combination of vitamin B2 and DNA under such condition, an electrochemically inactive supramolecular compound arised. Results: Under the experimental conditions selected, the decrease of oxidation peak currents had a linear relationship with DNA concentrations in the range of 0.01 to 0.1 mg/mL and 0.1 to 0.5 mg/mL, which could be used for measuring DNA concentration. Conclusion: The developed method is simple, rapid, economical and has good stability and reproducibility.
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