ZHAO Jiantong,ZHANG Lei,LIU Wenzhan, et al. Curcumin inhibits the proliferation of human renal cell carcinoma 786-O in nude mice via Erk1/2 signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(10): 735-738.
Abstract：Objective: To investigate the inhibitory effect of curcumin on 786-O cells and explore the potential mechanisms. Methods: Nude mice were randomly divided into two groups. Human kidney cancer cells 786-O were inoculated in nude mice. Intervention group mice were treated with curcumin and the control group mice were treated with PBS. The inhibition rate of tumor in the intervention group was determined. p-Erk1/2 and p-Akt expression was detected using Western blot and immunohistochemistry. Results: The average weight of the tumor of nude mice was (1.03±0.17)g in the intervention group and (2.46±0.48)g in the control group, the difference was significant (P=0.02). The tumor inhibition rate of mice treated with curcumin was 58.13%. Western blot and immunohistochemical staining showed that the phosphorylation level of Erk1/2 in intervention group was significantly lower than that in the control group (P<0.01), no significantly difference was observed in the expression of Erk1/2, Akt and p-Akt in the two groups (P>0.05). Conclusion: The results of this study show that curcumin can inhibit the growth of tumor in vivo and inhibit the phosphorylation of Erk1/2 and inhibit the proliferation of human renal carcinoma cell line 786-O in nude mice.
