Effects of propofol on potassium channels of S1 neurons in rats
HE Jiongce1, ZHANG Yu2, LIU Xingkui2, YU Tian2, HUANG Qianyu1
1.Department of Anesthesia and Perioperative Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Anaesthesiology, Zunyi Medical College, Zunyi, 563003
HE Jiongce,ZHANG Yu,LIU Xingkui, et al. Effects of propofol on potassium channels of S1 neurons in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(10): 713-717.
Abstract：Objective: To explore the effects of propofol on voltage-gated potassium channels of primary S1 neurons in rats. Methods: Whole-cell patch clamp technique was used to record potassium currents of neurons. After application with propofol at different concentrations (10-300 μmol/L) in ACSF, the currents of outward potassium currents (IA) and delayed rectifier potassium currents (IK) were recorded to make current-voltage curve, steady-state activation curve and account characteristic parameters. Results: Propofol inhibited IA of S1 neurons in rats in a dose-dependent manner. It also inhibited the activation of IA, shifted the activation curve towards the deperpolarzating potential. Propofol inhibited IK of S1 neurons of rats in a dose-dependent manner. But it did not affect activation of IK. Conclusion: The results of this study provide new vidence that propofol shows an inhibitory effect on voltage-gated potassium channels in the thalamocortical circuit, which may play a role in the mechanisms of propofol-induced general anesthesia.
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