piRNA在胃癌与正常胃黏膜组织中表达的比较

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摘要目的：分析piRNA在胃癌组织与正常胃黏膜组织中表达的差异性。方法：通过新一代高通量测序技术Solexa对胃癌组织和正常胃黏膜组织进行小分子RNA（sRNA）深度测序，并通过生物信息学分析胃癌组织与正常胃黏膜组织中piRNA的表达差异。根据分析结果选取4种piRNA（登录号分别为DQ570956、DQ575659、DQ594126和DQ597128），通过茎环反转录实时定量PCR（简称茎环RT-qPCR）技术验证其存在及其在胃癌组织和正常胃黏膜组织中的表达差异。结果：Solexa深度测序结果显示，正常胃黏膜与胃癌组织中测得的piRNA种类在各标本所测得所有sRNA种类中所占的比例差异无统计学意义（Z=0.835，P=0.678）；正常胃黏膜组织中测得的piRNA数量在各标本所测得sRNA总量中所占的比例高于胃癌组织，差异有统计学意义（Z= 2.167，P=0.042）；根据分析结果选取4种piRNA，经茎环RT-qPCR法检测显示，这4种piRNA在胃癌组织中的表达量显著低于正常胃黏膜组织，差异有统计学意义（P＜0.05）。结论：胃癌组织存在多种差异表达的piRNA，且在表达量上与正常胃黏膜组织存在显著性差异，本研究选取的4种piRNA可能参与胃癌的发生发展进程。

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	蒋佩佩
	蔡一奇
	王鹏飞
	陈孝冬
	金劲激
	胡畅远
	陈文静
	薛向阳
	张丽芳
	朱冠保

关键词 ：胃肿瘤, piRNA, 小分子RNA, 聚合酶链反应

Abstract：Objective: To analyze the differences of expression of piRNA in gastric carcinoma and normal gastric mucosa. Methods: The small RNA (sRNA) deep sequencing of gastric cancer tissue and normal gastric mucosa was performed by a new generation of high throughput sequencing technology, and the differential expression of piRNA was detected in gastric cancer tissue and normal gastric mucosa by follow-up bioinformatics. Four kinds of piRNAs (login ID: DQ570956, DQ575659, DQ594126 and DQ597128) were selected according to the results of the analysis, and their presence and their differences in gastric cancer tissues and normal gastric mucosa were verified by real-time quantitative PCR. Results: Solexa depth sequencing results showed that there was no significant difference in the proportion of sRNA measured in normal gastric mucosa and gastric cancer tissues (Z=0.835, P=0.678). The number of piRNAs measured in normal tissues was significantly higher than that in gastric cancer tissues (Z=2.167, P=0.042). Four piRNAs were selected according to the analysis results. The expression of that 4 piRNA in gastric cancer tissues was significantly lower in normal gastric mucosa (P<0.05). Conclusion: There are many differentially expressed piRNAs in gastric cancer tissues, and there is significant difference in the normal gastric mucosa. In this study, four piRNAs may be involved in the development and progression of gastric cancer, and the subsequent development of piRNA and gastric cancer of the pathogenesis of the study to provide experimental basis.

Key words： stomach neoplasms piRNA small RNA polymerase chain reaction

收稿日期: 2017-03-18

基金资助:国家自然科学基金资助项目（81372447）；浙江省自然科学基金资助项目（Y2100660）；温州市科技局科研基金资助项目（H20100028）。

通讯作者: 朱冠保，教授，Email：zgbwmc@126.com

作者简介: 蒋佩佩（1986-），女，浙江永嘉人，住院医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I10/708

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