转录因子SOX7对前列腺癌PC-3细胞生物学活性的影响及其机制

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摘要目的：探讨转录因子SOX7对前列腺癌PC-3细胞增殖、细胞周期、迁移和侵袭能力的影响及其机制。方法：采用脂质体法转染高表达SOX7重组质粒（pCDNA3.1-SOX7），以转染空质粒（Vector）作为对照，检测SOX7对PC-3细胞株增殖、细胞周期、迁移及侵袭的影响，并用Western blot法检测相关蛋白表达水平。结果：pCDNA3.1-SOX7显著抑制PC-3细胞增殖能力，并且诱导细胞周期发生G1期阻滞，同时，使Cylcin D1、E表达量明显下调。此外，pCDNA3.1-SOX7转染后，PC-3细胞迁移距离显著减少，穿透基质胶的细胞数量也显著降低，并且伴随着间质表型标记物MMP-2、MMP-9和N-cadherin蛋白表达减少，上皮标记物E-cadherin蛋白表达增加。结论：SOX7抑制前列腺癌PC-3细胞的增殖、周期、迁移及侵袭能力，其机制可能与调控这些细胞行为的相关蛋白表达有关。

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	郑斌
	吴齐全
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	王钢
	任雨
	祁洪刚
	朱伟智
	翁国斌

关键词 ： SOX7, 前列腺癌, 增殖, 迁移, 侵袭

Abstract：Objective: To investigate the effects and mechanisms of transcription factor SOX7 on PC-3 cells proliferation, cell cycle, cell migration and invasion. Methods: PC-3 cells were transfected with high-expressed SOX7 recombinant plasmid (pCDNA3.1-SOX7) and further detected the changes of cell proliferation, cell cycle, migration, invasion, and related protein expressions. Results: After transfection with pCDNA3.1-SOX7, significant inhibition effects was shown in the PC-3 cell proliferation and G1/S cell cycle arrest, which were accompanied with decreased expressions of cyclin D1 and E. Furthermore, transfection of pCDNA3.1-SOX7 markedly decreased the cell migration distances and invasion capacity, with a reduction of the mesenchymal markers (MMP-2, MMP-9 and N-cadherin), however, the epithelial marker E-cadherin was increased. Conclusion: SOX7 inhibits PC-3 cell proliferation, cell cycle, migration and invasion through regulation of its related protein expressions.

Key words： SOX7 prostate cancer proliferation migration invasion

收稿日期: 2016-12-27

基金资助:鄞州区科技计划项目；宁波市科技惠民项目（2017C50 058）。

通讯作者: 翁国斌，主任医师，教授，Email：ddwgb@aliyun.com

作者简介: 郑斌（1982-），男，浙江宁波人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I9/678

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