长链非编码RNA AL049452在结直肠癌组织中的表达及临床意义

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摘要目的：探索长链非编码RNA（lncRNA）AL049452在结直肠癌组织中的表达及临床意义。方法：收集84例手术切除的结直肠癌及相应的癌旁正常组织，qRT-PCR检测lncRNA-AL049452的表达水平，用Mann-Whitney U检验分析lncRNA-AL049452表达水平和结直肠癌患者临床病理特征的关系，同时分析其对结直肠癌患者的总生存时间及无病生存时间的影响。绘制ROC曲线评价lncRNA-AL049452诊断结直肠癌的效能。结果：结直肠癌患者肿瘤组织lncRNA-AL049452的表达水平明显高于癌旁正常组织（P=0.0035），肿瘤更大的结直肠癌患者其lncRNA-AL049452的表达量更高（P=0.024）。生存曲线分析显示，高表达lncRNA-AL049452的结直肠癌患者的无病生存时间较低表达组更短（log-rank=4.208，P=0.034）。ROC曲线分析显示，lncRNA-AL049452诊断结直肠癌的曲线下面积为0.723（95%CI=0.613～0.832，P＜0.001），敏感性和特异性分别为86.0%和51.2%。结论：肿瘤组织中高表达的lncRNA-AL049452可能参与了结直肠癌发生发展进程，是结直肠癌诊断和预后判断的新生物标记物。

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	孙祥威
	胡盈盈
	徐剑峰
	张良
	毛晨晨
	郭刚强
	黄关立
	沈贤
	薛向阳

关键词 ：长链非编码RNA, AL049452, 结直肠肿瘤, 生物学标记

Abstract：Objective: To explore the expression and clinical significance of lncRNA-AL049452 in colorectal cancer (CRC). Mothods: Eighty-four cases of CRC tissues and corresponding paracancerous tissues were collected. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of lncRNA-AL049452 in these tissue specimens. Correlation between the expression of lncRNA-AL049452 and clinicopathological features was analysed by Mann-Whitney U test. Kaplan-Meier survival curves was used to analysed overall survival time and disease-free survival time between different lncRNA-AL049452 levels. Receiver operating characteristic (ROC) curve was performed to observe the diagnostic value of lncRNA-AL049452 for colorectal cancer. Results: lncRNA-AL049452 expression level in CRC tissues was obviously higher than that in corresponding paracancerous tissues (P=0.0035). High expression of lncRNA-AL049452 indicated larger tumor size (P=0.024). The lncRNA-AL049452 high-expression group presented significantly lower disease-free survival rates than the low-expression group (log-rank=4.208, P=0.034). In addition, ROC curves analyse showed that the AUC of ROC curve was 0.723 (95%CI=0.613-0.832, P<0.001) with 86.0% sensitivity, 51.2% specificity. Conclusion: The high expression of lncRNA-AL049452 in tumor tissues may play an important role in the progression in CRC, and can serve as novel biomarker for the diagnosis and prognosis of CRC.

Key words： long non-coding RNA AL049452 colorectal neoplasms biological markers

收稿日期: 2016-07-31

基金资助:国家自然科学基金资助项目（81472308，31470891）；温州市科技计划项目（20150050）。

通讯作者: 薛向阳，副教授，Email：wzxxy001@163.com

作者简介: 孙祥威（1992-），男，浙江温州人，硕士生

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I8/566

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