Imagination of novel targeted molecular imaging probes in the early diagnosis of upper urinary tract epithelial carcinoma model
LIAO Guodong1, WANG Lijiang2, YU Weiwen1, ZHANG Jianshe3.
1.Department of Urology, Zhejiang Provincial People’s Hospital, Hangzhou, 310014; 2.Zhejiang University California International Nanosystems Institute, Zhejiang University, Hangzhou, 310027; 3.School of Basic Medical Science, Wenzhou Medical University, Wenzhou, 325035
LIAO Guodong,WANG Lijiang,YU Weiwen, et al. Imagination of novel targeted molecular imaging probes in the early diagnosis of upper urinary tract epithelial carcinoma model[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(8): 547-552.
Abstract：Objective: To investigate the characteristic and tumor targeting of up-converting phosphor (UCP) nano-probe in labeling orthotopic transplantation tumor of early upper urinary tract urothelial carcinoma in vivo. Methods: Water soluble UCP was synthesized by high temperature pyrolysis. Then, UCP was link to the pH (low) insertion peptide (pHLIP). Imaging of urothelial tumors in a tumor bearing animal model by pHLIP-UCP probe was proceeded using a small animal in vivo multispectral Maestro imaging system (CRI). Results: NaYF4:Yb3+, Er3+ upconversion nanocrystals were synthesized with good dispersibility, small particle size and high solution permeability. The upconversion luminescence signal of the UCP nanoprobe had stable luminescence and high sensitivity in the dark environment. Conclusion: The UCP nanoprobe can specifically bound to orthotopic transplanted tumor of upper urinary tract urothelial cancer in vivo, which shows the potential for in vivo imaging of early upper urinary tract epithelial carcinoma.
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