RUAN Liqi,HUANG Bo.. Wnt/β-catenin signaling pathway is involved in the osteoporosis of elder mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(7): 519-522.
Abstract：Objective: To evaluate the potential pole of Wnt/beta-catenin signaling pathway in the induction and development of osteoporosis within elder mice. Methods: Healthy ICR mice were randomly divided into three groups, including sham-operated group (n=10), orchiectomy (ORX) group (n=10) and ORX+androgen group (n=10). Testicles were removed from the mice within ORX group and ORX+androgen group. In sham-operated group, the surgery to separate the testicular capsula was performed and the testicles were intact. After surgery, all the mice were fed for 14 weeks freely with water and food under the same condition. The mice were subjected to the evaluation of the bone mineral density (BMD). The blood samples were collected from heart to examine the expression level of estrogen and androgen by an ELISA method. The bone tissues were obtained. The expression levels of β-catenin and Runx2 mRNA expression were determined by real-time PCR while the expression levels of β-catenin and Runx2 protein were evaluated by Western blot. Results: The BMD in the mice of ORX group was significantly decreased compared with NS group (P<0.05). The expression levels of both β-catenin and Runx2 in the mice of ORX group were also significantly decreased compared with sham-operated group (P<0.05). The mice in ORX+androgen group showed no significant difference from ORX group (P>0.05) in BMD. The treatment of androgen could enhance the expression levels of β-catenin and Runx2 in ORX group. Conclusion: Wnt/β-catenin signaling pathway is inhibited 14 weeks after orchiectomy in osteoporosis mice, which can be involved in the induction and development of the osteoporosis
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