ZHENG Qian qian,CHEN Liangliang,YE Xuesong. Establishment of the epilepsy model in vitro based on optical release of caged-glutamate[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(7): 510-513.
Abstract：Objective: In order to better study the internal mechanism of the seizure, the epilepsy model in vitro was established to simulate seizure discharge. Methods: Aiming at the drawbacks of the electrical stimulation and drugs intervention, a stimulation system of neurons based on optical release of caged-glutamate was developed. The hippocampal neurons cultured on the microelectrode array were used as the research object. The ultraviolet (UV) focused by the optical focus system was delivered to the bath solution contained cage-glutamate. Using a specific wavelength of UV to remove the caged function of photosensitive groups, glutamate was released. Results: The hippocampal neurons showed the normal potential when the UV did not work. The glutamate induced seizure discharge in the hippocampal neurons after the UV excitation. Conclusion: The epilepsy model in vitro was preliminarily set up. The model provides the rational model in vitro for the researches on the mechanism of the epilepsy.
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