RhoA基因在胰腺癌组织中的表达及其与肿瘤临床病理特征的关系

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摘要目的：探讨RhoA基因在胰腺癌组织中的表达情况及其与胰腺癌临床病理特征的关系。方法：应用实时荧光定量PCR与Western blot同步检测48例胰腺癌患者癌组织及对应癌旁组织中RhoA mRNA及蛋白的表达情况，分析RhoA基因的表达与胰腺癌组织临床病理参数的关系。结果：肿瘤组织中RhoA mRNA的表达量明显高于癌旁组织（t=3.669，P=0.0007），RhoA mRNA在胰腺癌中的表达水平与肿瘤分化程度、有无门脉及淋巴结转移、pTNM病理分期密切相关，在中、低分化肿瘤（t=2.447，P=0.006）、门静脉受侵犯（t=2.544，P=0.008）、淋巴结转移（t=2.351，P=0.041）和pTNM分期较晚（Ⅲ和Ⅳ期，t=2.667，P=0.025）的患者中明显升高。Western blot结果显示肿瘤组织中RhoA蛋白的表达水平明显高于癌旁组织（t=3.461，P=0.0003）；RhoA蛋白表达量与肿瘤侵袭能力以及肿瘤分期相关，RhoA蛋白的含量在中、低分化肿瘤（t=2.323，P=0.016）、门静脉受侵犯（t=2.119，P=0.036）、淋巴结转移（t=2.117，P=0.029）和pTNM分期较晚（Ⅲ和Ⅳ期，t=2.776，P=0.004）的患者中明显升高。结论：RhoA基因可能成为一种新的肿瘤标志物，用于判断肿瘤分化程度、转移能力和预后评估。

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	李金海
	戴华卫
	张海峰

关键词 ：胰腺肿瘤, 病理学, 临床, 基因表达, RhoA

Abstract：Objective: To investigate the expression of RhoA gene in tissues of pancreatic cancer and to discuss its significance during carcinogenesis and progression of tumor. Methods: Intratumor RhoA expression level was determined and compared with that in adjacent nontumorous pancreatic tissues using quantitative real time polymerase chain reaction and Western blot in 48 samples of pancreatic carcinoma. Results: The mRNA levels of RhoA were significantly higher in tumor tissues than that in the unaffected portions (t=3.669, P=0.0007). The expression of RhoA mRNA in the primary lesion was higher in patients with low and middle differentiation cancer (t=2.447, P=0.006), protal vein invasion (t=2.544, P=0.008), lymph node metastasis (t=2.351, P=0.041), and advanced pTNM stage (stage III/IV, t=2.667, P=0.025) than in those without. There was a significant difference between high RhoA protein levels in the tumor tissues and noncancerous tissues in pancreatic carcinoma patients(t=3.461, P=0.0003), the quantity of RhoA expression associated with tumor invasion and metastasis. There was a significant association between high tumor RhoA protein levels and low and middle differentiation cancer (t=2.323, P=0.016), and the presence of protal vein invasion (t=2.119, P=0.036), lymph node metastasis(t=2.117, P=0.029), and advanced pTNM stage (t=2.776, P=0.004). Conclusion: There is a significant correlation among RhoA expression, tumor stage, and metastasis. The expression of RhoA can be used as a good tumor marker for invasive and advanced pancreatic carcinoma as well as a prognosis predictor.

Key words： pancreatic neoplasms pathology, clinical gene expression RhoA

收稿日期: 2013-04-27

作者简介: 李金海（1983-），男，山东枣庄人，住院医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2014/V44/I2/130

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