芍药苷上调慢性压迫性损伤大鼠脊髓背角和背根节内Sirt1表达

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目的：观察芍药苷对慢性压迫性损伤（CCI）大鼠的疼痛行为和Sirt1在脊髓背角及背根节的表达变化。方法：选择雄性SD大鼠48只，按照设计随机分为假手术组（Sham组）、阴性对照组（SC组）、CCI组和芍药苷组。每组在术前和术后3、7和14 d检测大鼠的后足热缩足反射潜伏期（TWL）和机械缩足反射阈值（MWT）；选择在术后3、7和14 d的相同时间处死大鼠，取大鼠L4～5节段的脊髓和背根节组织备用，用real-time PCR检测Sirt1在组织内表达水平，采用Western blot法检测组织内Sirt1蛋白表达变化。结果：术后3、7和14 d，与Sham组比，CCI组TWL与MWT值均显著降低（P＜0.05），并且在术后3 d达到最小值；real-time PCR结果显示，与Sham组相比，术后7 d和14 d，CCI组脊髓背角及背根节的Sirt1基因表达显著降低（P＜0.05），与SC组对比，芍药苷组脊髓背角及背根节的Sirt1基因的表达上调（P＜0.05）；Western blot结果表明芍药苷组脊髓背角及背根节的Sirt1蛋白的表达随时间逐步升高，但在14 d时仍低于SC组（P＜0.05）。结论：芍药苷能够缓解神经病理性疾病引发的疼痛，其中上调脊髓背角和背根节Sirt1的表达是其可能机制。

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关键词 ：芍药苷, 慢性压迫性损伤, 脊髓背角, 背根节, Sirt1, 大鼠

Abstract：Objective: To observe the effect of paeoniflorin on pain behavior and the expression of Sirt1 in spinal dorsal horn and dorsal root ganglion in rats with chronic constriction injury. Methods: Forty-eight male SD rats were randomly divided into sham operation group, negative contract group (SC group), CCI group and paeoniflorin group. Heat shrinkable foot reflex latency (TWL) and mechanical paw withdrawal reflex threshold (MWT) were detected for rats in each group at 1 day before operation and postoperative 3, 7 and 14 days. Rats were sacrificed at 3, 7 and 14 days after surgery then L4-5 segment of spinal cord and dorsal root section organization was taken, and real-time PCR was used for expression of Sirt1, Western blot was used in the same time to detect the change of Sirt1 protein level. Results: At 3, 7 and 14 days after surgery, TWL and MWT of CCI group decreased significantly compared with sham group (P<0.05), and achieved the minimum value at the third day. Real-time PCR showed that the expression of Sirt1 of CCI group of spinal dorsal horn and dorsal root ganglia were significantly decreased at the 7th day and 14th day after surgery compared with sham group (P<0.05); the expression of Sirt1 of paeoniflorin group was upregulated compared with SC group (P<0.05); the results of Western blot also showed that the level of Sirt1 protein in the dorsal horn and dorsal root ganglion were gradually increased with time, and there was difference between the Sirt1 group and the SC group at 14th day. Conclusion: Paeoniflorin can alleviate by pain neuropathic disease caused, and the up regulation of the expression of Sirt1 protein in dorsal horn and dorsal root ganglion of the spinal cord is a possible mechanism.

Key words： paeoniflorin chronic constrictive injury spinal cord dorsal root ganglion Sirt1 rats

收稿日期: 2016-08-05

基金资助:浙江省中医药科学研究基金资助项目（2015ZB052）。

作者简介: 郑晖（1977-），男，浙江诸暨人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I6/435

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