MicroRNA-143/145对大鼠子宫内膜异位症的治疗作用

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摘要目的：研究microRNA（miRNA）-143/145对子宫内膜异位症（内异症）SD大鼠模型异位囊肿的治疗作用。方法：采用自体移植的方法建立大鼠内异症模型，并在病理组织学水平进行鉴定，选取18只建模成功的SD大鼠随机分为空白组（n=6）、阴性对照组（n=6）、miR-143/145类似物组（n=6），分别予以相应的处理，最后剖腹探查，用游标卡尺测量SD大鼠内异症模型异位囊肿的大小，比较3组间异位囊肿体积的差异，并比较3组间组织病理学的变化。结果：SD大鼠内异症建模成功率为84%；异位囊肿体积差异：治疗后空白组囊肿体积大小为（16.38±4.19）mm3，阴性对照组为（21.14±0.51）mm3，miR-143/145类似物组为（5.91±1.91）mm3，空白组与阴性对照组比差异无统计学意义（P＞0.05），而miR-143/145类似物组与阴性对照组比囊肿体积明显减小，差异有统计学意义（P＜0.01）。病理组织学变化：空白组与阴性对照组异位病灶病理组织学形态相似，而阴性对照组与miR-143/145类似物组相比，腺体数量减少，腺体萎缩，间质细胞排列杂乱。结论：miR-143/145可以使内异症的异位病灶萎缩而达到治疗效果。

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	段萍

关键词 ：子宫内膜异位症, miR-143/145, 大鼠, 治疗

Abstract：Objective: To investigate the therapeutic effect of miR-143/145 on endometriosis cysts in SD rat models. Methods: The model of endometriosis in SD rats was established by autologous transplantation, and was identified at the histopathological level. Eighteen SD rats were randomly divided into blank control group (n=6), negative control group (n=6) and miR-143/145 mimic group (n=6), were given the corresponding treatment, respectively. Finally laparotomy, and the size of ectopic cysts were measured by vernier caliper, and the volume of ectopic cysts were compared among the three groups. The histopathological changes of the three groups were also compared. Results: The successful rate of modeling of endometriosis in SD rats was 84%. After treatment, the difference in the volume of ectopic cysts in each group: the volume of ectopic cysts in control group was (16.38±4.19) mm3, negative control group was (21.14±0.51) mm3, miR-143/145 mimic group was (5.91±1.91) mm3. There was no significant difference between the blank control group and the negative control group (P>0.05), however, the volume of ectopic cysts in the miR-143/145 mimic group was significantly reduced compared with negative control group (P<0.01). The histopathological changes: the histopathological morphology of ectopic cyst in blank control group and negative control group was similar, but in miR-143/145 mimic group the number of glands was decreased, the glandular atrophy, and the arrangement of stromal cell were messy compared with the mimic NC group. Conclusion: MiR-143/145 can atrophy ectopic cysts of endometriosis and achieve therapeutic effect.

Key words： endometriosis miR-143/145 rats treatment

收稿日期: 2016-11-08

基金资助:国家自然科学基金资助项目（8140060904）；浙江省卫生厅平台重点资助项目（2013RCA035）；温州市科技局科研基金资助项目（Y20100175）；浙江省科技厅公益项目（2014C37003）。

通讯作者: 段萍，主任医师，Email：dppddpp@126.com

作者简介: 梁宗文（1990-），女，甘肃白银人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I6/397

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