脑梗死患者凝血因子Ⅻ Exon1-46C/T基因多态性

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摘要目的：探讨凝血因子XII（FXII）基因Exon1-46C/T多态性与脑梗死的相关性及对机体纤溶功能的影响。方法：采用直接测序法测定脑梗死组（60例）与正常对照组（60例）的FXII基因46C/T多态性，同时采用自动血凝仪测定FXII活性（FXII:C）、纤溶酶原活性（PLG:A）、纤维蛋白原（FIB）及D-二聚体（D-D）含量等指标。结果：脑梗死组比正常对照组中46TT明显升高，且2组间多态性分布比较差异有统计学意义（P＜0.05）。通过基因型单因素分析，脑梗死患者中T等位基因危险性是C等位基因的1.865倍，TT基因型危险性为结合（CC+CT）基因型的3.021倍。与正常对照组比较，脑梗死组46TT基因型FXII:C明显下降，D-D含量升高，PLG:A下降（P＜0.05），但脑梗死患者FXII多态性各型之间的D-D指标差异无统计学意义（P＞0.05）。结论：FXII基因46TT多态性与脑梗死的发生有关联，其可导致FXII活性明显下降，致使机体的纤溶功能受影响。

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关键词 ：脑梗死, 凝血因子XII, 多态性, 纤溶功能

Abstract：Objective: To investigate the correlation and fibrinolysis influence of factor XII gene (FXII) polymorphism on the patients with cerebral infarction. Methods: Direct sequencing was used to analyze the 46 polymorphism at promoter regions in factor XII gene among cerebral infarction patients and healthy controls. Meanwhile, the activity of plasma factor XII (FXII:C), fibrinogen (FIB), D-dimer (D-D) and the activity of plasminogen (PLG:A) were measured. Results: The rate of 46TT of factor XII gene (FXII) polymorphism in patients with cerebral infarction was higher than that in healthy controls, and the differences on the distribution were statistically significant (P<0.05). By analysis of genotype single factor, the risk of T genotype was higher 1.865 times than that of C genetype and the risk of TT genotype was higher 3.021 times than that of (CC+CT) unite genotype in the cerebral infarction patients. Compared with the healthy controls, the FXII:C and PLG:A were obviously lower and D-D were higher in 46TT group (P<0.05), and there were no differences of D-D in these groups. Conclusion: The FXII gene 46TT polymorphism is obviously increased in patients with cerebral infarction, and it can lead to evidently decreased the activity of FXII, then affects the body’s fibrinolysis.

Key words： cerebral infarction factor XII polymorphism fibrinolysis

收稿日期: 2016-05-17

基金资助:温州市科技计划项目（Y20150306）。

作者简介: 徐琦煜（1984-），女，浙江温州人，主管技师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I5/372

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