胰岛素样生长因子结合蛋白7对胃癌细胞株SGC-7901细胞周期的影响

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摘要目的：通过体外实验测定胰岛素样生长因子结合蛋白7（IGFBP7）对人胃癌细胞株SGC-7901细胞周期的影响。方法：构建pCMV-XL4-IGFBP7质粒，将SGC-7901细胞随机分为pCMV-XL4-IGFBP7实验组和pCMV-XL4空质粒对照组。不同SGC-7901细胞进行相应质粒转染后，通过MTS法检测IGFBP7基因表达上调对SGC-7901细胞增殖活性的影响，采用流式细胞仪检测SGC-7901细胞周期。结果：pCMV-XL4-IGFBP7实验组的SGC-7901细胞处于G1期比率高于pCMV-XL4空质粒对照组，而处于G2/M的细胞比率低于pCMV-XL4空质粒对照组（P＜0.05）；用MTS检测2组转染后的SGC-7901细胞存活率差异无统计学意义（P＞0.05）。结论：IGFBP7对SGC-7901细胞生物行为学的影响主要表现为抑制其细胞周期。

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关键词 ：胰岛素样生长因子结合蛋白7, 胃肿瘤, 细胞增殖, 细胞周期

Abstract：Objective: To investigate the effect of insulin-like growth factor binding protein 7 on gastric cancer SGC-7901 cells and its mechanisms. Methods: The recombinant expressing plasmid pCMV-XL4-IGFBP7 was constructed and transfected into SGC-7901 cells. Empty plasmid transfection was regarded the control. Cell proliferation was analyzed by MTS assay; cell apoptosis was detected by flow cytometer. Results: Compared with control group, the rate of SGC-7901 cell of pCMV-XL4-IGFBP7 group on G1 level was higher; while the rate of SGC-7901 cell of pCMV-XL4-IGFBP7 group on G2/M was lower than the control group. The proliferation of SGC-7901 cells had no significant change compared with the control group. Conclusion: IGFBP7 may inhibit gastric cancer SGC-7901 cells through influencing the cell cycle.

Key words： insulin-like growth factor binding protein-7 gastric tumor cell proliferation cell cycle

收稿日期: 2016-09-30

基金资助:绍兴市科技计划项目（2015B70041）。

作者简介: 胡剑浩（1978-），男，浙江绍兴人，副主任医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I4/296

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