GU Xuejiang,WU Senmin,Hong Yilian, et al. Compartmental imbalance and aberrant immune function of dendritic cells in peripheral blood from Graves’ disease[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(4): 239-243.
Abstract：Objective: To assess the imbalance of dendritic cells (DC) in the peripheral blood from patients with Graves’ disease (GD), and the influence on the differentiation of T helpler cells (Th). Methods: In total, 36 untreated GD patients and 21 healthy individuals were enrolled in the study. The circulating DC levels, including plasmacytoid DC (pDC) and myeloid DC (mDC), were identified by FACS. In further, pDC and mDC isolated by MACS were co-culture with the naïve T cells to investigate the role on proliferation and differentiation of Th cells. Results: A significant increase in the percentage of circulating DC and pDC subset was found in GD patients compared with healthy individuals, and GD-derived pDCs were conferred the ability to induce the proliferation of T cells as well as the polarized differentiation of Th1 in an IFN-α-dependent manner. Conclusion: The elevated circulating pDCs in GD patients can play a role on the differentiation of Th1 in IFN-α-dependent manner, and contribute significantly to the pathogenesis.
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