CHEN Miaomiao,WANG Ying,ZHANG Wenwen, et al. Effects of enhanced expression of S100A9 mediated by recombinant adenovirus on proliferation and apoptosis of squamous cervical cancer cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(4): 235-238.
Abstract：Objective: To construct the recombinant adenovirus carrying S100A9 gene, and to explore its effect on the proliferation and apoptosis of squamous cervical cancer cells. Methods: The shuttle plasmid pHBAd-MCMV-RFP-S100A9 was constructed and co-transfected into HEK293 cells with backbone plasmid pHBAd-BHG to produce the recombinant adenovirus Ad-S100A9. Then the C-33A cells were infected with Ad-S100A9, cells infected with Ad-RFP as negative control group, and cells without infection as blank group. S100A9 protein expression was examined by western blot. CCK-8 and flow cytometry were carried out to examine the effects of S100A9 over-expression cell proliferation and apoptosis of C-33A cells, respectively. Results: Ad-S100A9 was successfully constructed. Ad-S100A9 infection significantly enhanced the expression of S100A9 in C-33A cells, and effectively promoted cell proliferation (P<0.05), but had no effect on cell apoptosis. Conclusion: Ad-S100A9 can mediate S100A9 over-expression and promote cell proliferation of squamous cervical cancer cells.
[1] LEANDERSON T, LIBERG D, IVARS F. S100A9 as a pharmacological target molecule in inflammation and cancer [J]. Endocr Metab Immune Disord Drug Targets, 2015, 15 (2): 97-104.
[2] GUNALDI M, OKUTURLAR Y, GEDIKBASI A, et al. Diagnostic importance of S100A9 and S100A12 in breast cancer[J]. Biomed Pharmacother, 2015, 76: 52-56.
[3] RECKENBEIL J, KRAUS D, PROBSTMEIER R, et al. Cellular distribution and gene expression pattern of metastasin (S100A4), calgranulin A (S100A8), and calgranulin B (S100A9) in oral lesions as markers for molecular pathology[J]. Cancer Invest, 2016, 34(6): 246-254.
[4] ZHU X, JIN L, ZOU S, et al. Immunohistochemical expression of RAGE and its ligand (S100A9) in cervical lesions[J]. Cell Biochem Biophys, 2013, 66(3): 843-850.
[5] TORRE L A, BRAY F, SIEGEL R L, et al. Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2): 87-108.
[6] 薛纪森, 陈骋, 朱华, 等. IL-17在高危型HPV阳性宫颈癌中的表达[J]. 温州医科大学学报, 2016, 46(1): 24-27, 32.
[7] DUAN L, WU R, YE L, et al. S100A8 and S100A9 are associated with colorectal carcinoma progression and contribute to colorectal carcinoma cell survival and migration via Wnt/β-catenin pathway[J]. PLoS One, 2013, 8(4): e62092.
[8] Choi D K, Li Z J, Chang I K, et al. Clinicopathological roles of S100A8 and S100A9 in cutaneous squamous cell carcinoma in vivo and in vitro[J]. Arch Dermatol Res, 2014,306(5): 489-496.
[9] WU R, DUAN L, YE L, et al. S100A9 promotes the proliferation and invasion of HepG2 hepatocellular carcinoma cells via the activation of the MAPK signaling pathway[J]. Int J Oncol, 2013, 42(3): 1001-1010.
