miR-195在乳腺癌患者血浆中的表达及其临床意义

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摘要目的：探讨miR-195在乳腺浸润性导管癌患者术前、术后2周血浆中的表达及其与乳腺癌临床病理特征的相关性。方法：以miR-16为内参，采用茎环RT-qPCR方法检测48例术前、术后2周乳腺浸润性导管癌患者及35例健康对照者血浆中miR-195的表达，并分析其表达与乳腺浸润性导管癌临床病理指标的关系。结果：乳腺浸润性导管癌患者血浆中miR-195的表达较健康对照者明显升高（P＜0.05），术后2周表达明显降低至健康对照者水平。ROC曲线显示，血浆miR-195评价乳腺浸润性导管癌的敏感性和特异性可达94.4%和68.8%。与临床特征相关性分析统计未见血浆miR-195表达与乳腺浸润性导管癌肿块大小、雌激素受体（ER）、孕激素受体（PR）、人类表皮生长因子受体-2（Her-2）状态及淋巴结转移状态等临床病理特征有明显相关（P＞0.05）。结论：血浆中miR-195的异常表达可作为乳腺癌诊断的新的肿瘤标志物。

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	许践刚1
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关键词 ：微RNAs, miR-195, 乳腺肿瘤

Abstract：Objective: To explore the expression of miR-195 in plasma of patients with breast cancer in pre-operative and postoperative and two weeks and it correlation with clinicopathologic features of breast cancer. Methods: By normalized to miR-16, the expressions of miR-195 in 48 invasive ductal carcinoma of the breast cancer at preoperative and postoperative 2 weeks and 35 healthy controls were examined by stem-loop real-time RT-qPCR method. The relationship between miR-195 expression and clinicopathological characteristics were further analyzed. Results: Expression of miR-195 in plasma of patients with breast cancer was obviously higher than that in the healthy controls (P<0.05). At 2 weeks postopratively, a significant decrease of plasma miR-195 was observed, reaching levels of the healthy control subjects. ROC curve showed plasma levels of miR-195 could detect individuals with breast cancer with 94.4% sensitivity and 68.8% specificity. Correlative analysis of clinical features showed that there was no significant association between plasma miR-195 expression and the size of tumor, the state of ER, PR, lymph node metastasis and Her-2 receptor of breast cancer. Conclusion: Aberrant expression of miR-195 in plasma of breast cancer patients has potential use as novel noninvasive biomarkers for breast cancer.

Key words： microRNA miR-195 breast tumor

收稿日期: 2013-09-15

基金资助:温州市科技计划项目（Y20110119）

通讯作者: 沈洁，主任医师，Email：Linfeng0316@aliyun.com。

作者简介: 许践刚（1973-），男，浙江泰顺人，副主任医师，医学硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2014/V44/I2/105

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