CD117和Sox10在恶性黑色素瘤中的表达及意义

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摘要目的：分析黏膜、肢端和非肢端皮肤恶性黑色素瘤（MM）中CD117和Sox10表达以及c-kit基因扩增情况，探讨其与临床病理的关系。方法：PCR法检测黏膜、肢端和非肢端MM中c-kit基因的扩增量，并以色素痣作为对照。免疫组织化学法检测黏膜、肢端和非肢端MM中CD117的表达量，并与色素痣进行对照。免疫组织化学法检测黏膜、肢端和非肢端MM中Sox10、S-100和HMB45的表达，比较分析各组阳性细胞数及总的阳性率差异。结果：黏膜、肢端和非肢端MM中c-kit基因扩增量显著大于色素痣，差异有统计学意义（P＜0.05）。黏膜、肢端、非肢端MM和色素痣中CD117阳性率分别为78.5%、73.6%、73.3%和25.0%，3组MM阳性率均高于色素痣（P＜0.05）。3组MM中Sox10的阳性细胞数均高于S-100和HMB45，差异有统计学意义（P＜0.05）；Sox10、S-100和HMB45在MM中的阳性率分别为100.0%、87.5%和70.8%，差异有统计学意义（P＜0.05）。结论：c-kit基因扩增及CD117蛋白表达水平增高不仅可作为MM与色素痣的鉴别诊断标志，同时也为MM的靶向治疗提供靶点；Sox10阳性率可作为黑色素细胞起源的肿瘤诊断标志。

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	温莉虹
	林瑞泼
	陈阿德
	黄兆浪
	郑向阳
	李秧秧
	黄卡特
	陈国荣

关键词 ：黑色素瘤, CD117, c-kit, Sox10, 免疫组织化学

Abstract：Objective: To analyze the value of CD117, Sox10 expression and c-kit gene amplification in mucous, acral and non-acral melanoma and explore the relationship between it and clinical pathology. Methods: The gene levels of c-kit in mucosal, acral and non acral melanoma were detected by PCR, with pigmented nevus as control. The protein expressions of CD117, sox10, 1-100 and HMB45 were detected by immunohistochemistry. Results: The expression of c-kit gene from the three MM groups was higher than that of pigmented nevus (P<0.05). The immunohistochemical results demonstrated that the number of positive cells of CD117 from the three MM groups was marginally higher than that of pigmented nevus (P<0.05). The number of positive cells of Sox10 in the three MM groups was higher than that of S-100 and HMB45 (P<0.05). The positive rate of CD117 in mucosal, acral and non acral MM was 78.5%, 73.6% and 73.3% respectively, while 25% in pigmented nevus control (P<0.05). The positive rate of Sox10, S-100 and HMB45 in MM was 100.0%, 87.5% and 70.8% respectively (P<0.05). Conclusion: C-kit gene amplification and its downstream transcription protein CD117 enhanced positive expression can be applied as clinical potential therapy target. The positive rate of Sox10 is higher than S-100 and HMB45, and may be applied as tumor differentiation marker for origins of melanocytes.

Key words： melanoma CD117 c-kit Sox10 immunohistochemistry

收稿日期: 2016-09-18

基金资助:苍南县科技计划项目（2015S05）；温州市科技局科研基金资助项目（Y20130001）；浙江省科技厅国际合作项目（2013C24031）。

通讯作者: 陈国荣，教授，主任医师，硕士生导师，Email：chengr1978@aliyun.com。

作者简介: 温莉虹（1980-），女，浙江苍南人，副主任医师。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I3/197

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