CHEN Zhe,ZHAO Shishi,LIN Tingting, et al. Long-chain lipid emulsion reverse the decrease of cardiomyocyte membrane fluidity induced by bupivacaine[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(3): 178-181.
Abstract：Objective: To study the effects of long-chain lipid emulsion on the changes of membrane fluidity induced by bupivacaine in H9c2 cardiomyocyte. Methods: By the combination of confocal laser scanning microscope and fluorescence recovery after photobleaching, the effects of long-chain lipid emulsion and bupivacaine on the membrane fluidity in H9c2 cardiomyocyte, whose membrane phospholipid was labelled with NBD-C6-HPC specific fluorescent probes, were determined. Results: Comparing with the Con group, the LCT group showed no significant difference in membrane fluidity. The Bu-1000 group showed a decreased recovery ratio when compared with the Con group (P<0.01), LCT group (P<0.01) and Bu-100 group (P<0.01), and a decreased diffusion coefficient when compared with the Con group (P<0.05) and the LCT group (P<0.01). Compared with Bu-1000 group, the Bu-1000+LCT group showed an increased recovery ratio (P<0.05) and an increased diffusion coefficient (P<0.01). Compared with Bu-100 group, the Bu-100+LCT group showed an increased diffusion coefficient (P<0.01). Conclusion: Long-chain lipid emulsion can reverse the decrease of membrane fluidity induced by bupivacaine in H9c2 cardiomyocyte.
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