CHEN Hongfei,CAI Xixi,XIA Fangfang, et al. The effect of epinephrine on reversing asystole induced by different concentrations of bupivacaine in isolated rat hearts[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(2): 124-127.
Abstract：Objective: To determine the effect of epinephrine on reversing asystole induced by different bupivacaine concentrations in isolated rat hearts. Methods: Thirty-six male Sprague-Dawley rats were randomly divided into six groups (n=6), then the isolated hearts asystole model induced by bupivacaine were constructed. The control group was perfused with 0.15 μg/mL epinephrine; B10, B20, B30, B40 and B50 groups were perfused with 10, 20, 30, 40 or 50 µmol/L bupivacaine and 0.15 μg/mL epinephrine respectively. The values of heart rate (HR) and rate-pressure product (RPP) were recorded after recovery of heartbeat. Results: Isolated hearts in each group achieved recovery of heartbeat but the B50 group. The rank order of HR and RPP during 15 minutes after recovery of heartbeat were the control group>the B10 group>the B20 group>the B30 group>the B40 group (P<0.01). The ratio of the maximum RPP during recovery to baseline value (y) and the concentrations of bupivacaine (x) showed a linear relationship. The linear regression equation was y=-2.385x+114.5, pearson correlation coefficient r=-0.993. Conclusion: In the isolated heart level, the resuscitation effect of epinephrine and the concentration of bupivacaine are negatively correlated. While the bupivacaine concentration is 50 µmol/L, the resuscitation effect of epinephrine has been limited.
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