成纤维细胞激活蛋白α在神经胶质瘤中的表达及临床意义

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摘要目的：探讨成纤维细胞激活蛋白α（FAP-α）在神经胶质瘤组织中的表达及其与临床病理特征和预后的关系。方法：应用免疫组织化学法检测33例神经胶质瘤和7例正常对照脑组织中FAP-α蛋白的表达，并结合临床病理资料进行分析。同时对所有病例进行随访，分析FAP-α表达与患者预后的关系。进一步检索脑肿瘤分子数据库（Rembrandt）探讨FAP mRNA表达与预后的关系。结果：低级别胶质瘤和高级别胶质瘤之间FAP-α蛋白的积分光密度值（IOD）差异有统计学意义（P＜0.01），且与胶质瘤病理分级存在正相关（r=0.673，P＜0.01）。7例正常脑组织FAP-α蛋白不表达或表达弱阳性，与低级别胶质瘤（P＜0.05）和高级别胶质瘤（P＜0.01）比较差异有统计学意义。FAP-α蛋白的表达强度与患者性别、肿瘤大小、年龄以及手术切除程度无明显相关性（P＞0.05）。生存分析显示FAP-α低表达组和高表达组生存率差异有统计学意义（P＜0.01），FAP-α的表达是影响胶质瘤患者预后的独立因素（P＜0.05）。检索脑肿瘤分子数据库，发现FAP mRNA低表达预后明显好于高表达（P＜0.01）。结论：FAP-α在神经胶质瘤中存在表达，且与神经胶质瘤的病理分级正相关，其高表达可能提示不良预后。

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	陈渊
	苏志鹏
	金涌
	郑伟明

关键词 ：成纤维细胞激活蛋白&alpha, ；神经胶质瘤；免疫组织化学；预后

Abstract：Objective: To explore the expression of FAP-α in human gliomas, and its relationship between clinical characteristics and prognosis. Methods: Expression level of FAP-α protein was detected with immunohistochemistry in 33 cases of human gliomas and 7 cases of normal brain tissue, followed by subsequent clinicopathological correlative studies. Meanwhile, all patients were followed up, the relationship between the expression level of FAP-α and the glioma prognosis were studied. Furthermore, brain tumor molecular database (Rembrandt) was searched to investigate the relationship between the FAPmRNA expression and prognosis. Results: Comparing the expression intensities (IOD) of FAP-α protein in low-grade gliomas with those in high-grade gliomas, it was significantly different (P<0.01); the expressions of FAP-α and the pathological grades of glioma were positively correlated (r=0.673, P<0.01). There was low or no expression in 7 cases of normal brain tissue; Comparing with low grade glioma (P<0.05) and high grade glioma (P<0.01), there was significantly difference. The FAP-α expression was not significantly correlated with gender or tumor size or surgical degree and age (P>0.05). By survival analysis, overall survival time was significantly difference between the Low FAP-α and High FAP-α (P<0.01), and the expression of FAP-α was a independent prognostic factor in human gliomas (P<0.05). According to the results obtained from Rembrandt, patients with high FAP mRNA-expressing glioma showed statistically poorer prognoses compared with patients with low FAP mRNA-expressing glioma. Conclusion: FAP-α is expressed in human gliomas, which is positively correlated with the pathological grade of gliomas, and its high expression may suggest a poor prognosis.

Key words： fibroblast activation protein α glioma immunohistochemistry prognosis

收稿日期: 2016-05-17

基金资助:浙江省外科学重中之重学科开放基金资助项目（kfjj 2011006）。

通讯作者: 郑伟明，主任医师，硕士生导师，Email：zhwm61@ 126.com。

作者简介: 陈渊（1988-），男，浙江台州人，住院医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2017/V47/I2/115

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