Clinical application of LAMP and Xpert Mtb/RIF in early detection of pulmonary tuberculosis infection
ZHANG Weiyang1, ZHONG Jianping1, YANG Guobiao1, JIN Faxiang2, XU Wenfang2, WANG Huajun1.
1.Department of Infectious Diseases, Shaoxing Municipal Hospital, Shaoxing, 312000; 2.Department of Clinical Laboratory, Shaoxing Municipal Hospital, Shaoxing, 312000
ZHANG Weiyang,ZHONG Jianping,YANG Guobiao, et al. Clinical application of LAMP and Xpert Mtb/RIF in early detection of pulmonary tuberculosis infection[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(1): 61-63.
Abstract：Objective: To compare the clinical application of DNA loop mediated isothermal amplification (LAMP) technology and Xpert MTB/RIF in early detection of the infectious source of pulmonary tuberculosis. Methods: Three hundred TB sputum samples were detected using Xpert Mtb/RIF detection, MGIT liquid culture and LAMP technology, of which 100 cases of smear-positive specimens, 200 cases of smear-negative specimens. Results: In 100 cases of TB smear-positive specimens, the positive rate was 96.0% with LAMP technology, and the positive rate was 97.0% with Xpert Mtb/RIF detection (P>0.05). In 200 cases of smear-negative specimens, the positive rate was 53.5% with LAMP technology, and the positive rate was 25.0% with Xpert Mtb/RIF detection (P<0.01). Conclusion: In the diagnosis of smear positive specimens, the sensitivity of LAMP technology and Mtb RIF/Xpert was similar. In the diagnosis of smear negative specimens, LAMP technology is better than Mtb Xpert/RIF.
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