Stablishment of vascular endothelial cell injury model induced by high methionine feed diet in rats
REN Xiaoli1, Yang Bo2, CHEN Xiwen1, LOU Yongliang3.
1.Laboratory Animal Research Center, Wenzhou Medical University, Whenzou, 325035; 2.School of Environment Science and Public Health, Wenzhou Medical University, Whenzou, 325035; 3.School of Laboratory Medicine and Life Science, Wenzhou Medical University, Whenzou, 325035
REN Xiaoli,Yang Bo,CHEN Xiwen, et al. Stablishment of vascular endothelial cell injury model induced by high methionine feed diet in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(1): 47-51.
Abstract：Objective: To investigate the effects of the diet rich in different proportions of methionine on endothelial injury in sprague-dawley (SD) rats. Methods: Male SD rats were randomly divided into the normal diet group (CR), 1% methionine loading diet group (M1 R), 3% methionine loading diet group (M3 R) and 5% methionine loading diet group (M5 R), and blood in tail vein were drawn at 0, 2nd, 4th, 6th and 8th week, respectively. After 8 weeks, serum concentrations of homocysteine (Hcy) were measured, and the endothelial ultrastructure changes in aorta wall were observed using scanning electron microscope. Results: A significant interaction between high methionine diet and feeding duration on endothelial injury was found (P<0.05), and serum levels of Hcy were significantly increased in rats fed with high methionine diet (P<0.05), with feeding duration increasing. Compared with CR, serum level of Hcy in M3 and M5 R was significantly higher at 2nd week (P<0.01), whereas serum Hcy in M1 R was significantly higher at 4th week (P<0.05). Endothelial cells in M1 R were imperfectly unanimous in size, and the cell surface shrinked. Endothelium in M3 R and M5 R were found to be damaged by worms kinds of harming typical, with enclose wall thrombus and the fat deposition. Conclusion: High dietary intake of methionine is significantly associated with endothelial injury, which suggests that a method setting up endothelial injury animal models would be established.
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