Dynamic changes of TNF-α and gelatinase on murine viral myocarditis model
ZHOU Aihua1, YUE Yayan2, JIN Zengyou1, PAN Lulu3, ZHANG Yingying1, WANG Dan1, QIAN Yan1, ZHANG Yuanhai3, WU Rongzhou3, CHU Maoping3.
1.Department of Pediatrics, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Pediatric Cardiovascular disease, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027
RUAN Miaohua,WANG Dan,WANG Kai, et al. Dynamic changes of TNF-α and gelatinase on murine viral myocarditis model[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(12): 896-900.
Abstract：Objective: To explore the dynarnic changes and its meaning of TNF-α and gelatinase on the murine viral myocarditis model. Methods: Six-week-old inbred male mice were randomly assigned to control (n=35) and myocarditis group (n=60). The myocarditis and the control groups were inoculated intraperitoneally with 0.1 mL 10-5.69 TCID50/mL CVB3 or vehicle (PBS) alone respectively. Eight mice were sacrificed at 4th day, 10th day, 21th day and 30th day after injection. The serum content of TNF-α was measured by ELISA. The myocardial levels of MMP-2 and MMP-9 were determined by Western blot, MMP-2 mRNA and MMP-9 mRNA were measured by RT-PCR. Results: Compared with the control group, the protein and mRNA expression of MMP-2 and MMP-9 in the myocardium and the serum contents of TNF-α were significantly increased in myocarditis group (P<0.05). Conclusion: The expression of TNF-α and its downstream production MMP-2 and MMP-9 are increased in mice with acute viral myocarditis, and it may be involved in the pathogenesis of viral myocarditis.
