Investigation of the adjusting effect of WNK1 kinase on Maxi K channel
SHI Zhen1, ZHOU Lina2, ZHANG Yiqian1, WANG Dexuan3, ZHUANG Jieqiu3.
1.Department of Nephrology, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Nephrology, Wenzhou People’s Hospital, Wenzhou, 325000; 3.Department of Pediatric Nephrology, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325027
SHI Zhen,ZHOU Lina,ZHANG Yiqian, et al. Investigation of the adjusting effect of WNK1 kinase on Maxi K channel[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(12): 869-872.
Abstract：Objective: To investigate the adjusting effect of WNK1 kinase on Maxi K channel in HEK-293T cells. Methods: The DNA plasmid carrying WNK1 and Maxi K gene was transfected into the HEK-293T cells, to observe the distribution and total protein expression of Maxi K in the HEK-293T cells via immunoflu-orescence microscopy and Western blot. Results: Immunofluorescence microscopic study showed that Maxi K expression was markedly increased in experimental group compared with the control group. Western blotting analysis showed that total Maxi K protein level was markedly increased in the presence of experimental group 1 and experimental group 2 compared with the control group (P<0.01). The total Maxi K protein level was markedly increased in experimental group 2 compared with the experimental group 1 (P<0.05). Conclusion: WNK1 can upregulate total protein expression of Maxi K, and the effect is enhanced with the increasing amount of WNK1 DNA, showing that the effect was dose-dependent.
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