条件性刺激诱导大鼠建立异丙酚复吸行为

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摘要目的：利用条件性刺激诱导大鼠建立异丙酚复吸行为。方法：在固定比率（FR=1）程序下连续训练14 d建立自身给药，大鼠24只，随机分为3组（n=8）：戒断1 d（R1）组、戒断14 d（R14）组和戒断30 d（R30）组。大鼠进行复吸测试2 h（前1 h为实验笼空间环境诱导，后1 h为条件性刺激诱导），记录动物在2 h内的有效或无效鼻触数。结果：异丙酚自身给药训练14 d，建立了稳定的异丙酚静脉自身给药行为。和无效鼻触相比，有效鼻触数明显增加，差异有统计学意义（P＜0.05）。与R1组比，R14组有效鼻触数显著升高，差异有统计学意义（P＜0.01），R30组有效鼻触数亦升高，但差异无统计学意义（P=0.057）。条件性刺激诱导的有效鼻触反应比实验笼空间环境的诱导作用更强烈（P＜0.05）。结论：条件性刺激可以诱导大鼠建立异丙酚复吸行为，异丙酚具有明显的复吸特性。

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	王本福
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	连庆泉

关键词 ：异丙酚, 复吸, 环境线索, 自身给药

Abstract：Objective: To induce rat establishing propofol relapse behavior, conditioned cues was utilized. Methods: Rats received propofol self-administration 3 h session per day for a total of 14 days under FR=1 (fixed- ratio=1) schedule. Twenty-four rats well trained for propofol self-administration were divided into 3 groups (n=8): Relapse 1 day (R1), Relapse 14 days (R14) and Relapse 30 days (R30). After withdrawal, those rats reintroduce to training-chambers for consecutive 1 hour relapse testing induced by contextual and contingent discreet conditioned cues respectively. Results: Stable propofol self-administration was established after 14 days training sessions. Compared to inactive nose-poke response, the number of active nose-poke response increased significantly (P<0.01). Compared to active nose-poke response of R1, the number of active nose-poke response of R14 increased significantly after 14 withdrawal days (P<0.01). And the number of active nose-poke response of R30 also increased after withdrawal 30 days, but there was no statistic difference compared with that of R1 (P=0.057). The number of active nose-poke response of contingent discreet conditioned cues was strongly higher than that of contextual (P<0.01). Conclusion: Propofol relapse can be induced by drug-related environmental cues, and propofol has obvious characteristics of relapse.

Key words： propofol relapse environmental cues self-administration

收稿日期: 2016-01-15

基金资助:国家自然科学基金资助项目（81271469）；温州市科技局科研基金资助项目（Y20140692，H20100060）；浙江省医药卫生科技项目（2014KYB161）。

通讯作者: 连庆泉，主任医生，教授，Email：lianqingquanmz@163.com。

作者简介: 王本福（1980-），男，浙江温州人，主治医师，硕士。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2016/V46/I12/865

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