Analysis of the characteristics of gene seguence of human cytomegalovirus US31 and predication of B cell epitopes
GUO Gangqiang1, XIE Shangdan2, YE Sisi1, SUN Xiangwei3, ZHANG Liang3, ZHANG Lifang1, XUE Xiangyang1
1.Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, 325035; 2.The Second Clinical College of Wenzhou Medical University, Wenzhou, 325035; 3.Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
GUO Gangqiang,XIE Shangdan,YE Sisi, et al. Analysis of the characteristics of gene seguence of human cytomegalovirus US31 and predication of B cell epitopes[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(11): 781-786.
Abstract：Objective: To analyze the sequence of human cytomegalovirus US31 gene and predict the B cell epitopes of US31 protein. Methods: The hydrophilicity, accessibility, antigenicity and flexibility index were used to predict the potential B cell epitopes of protein based on the US31 amino acid sequence. Results: Most amino acid sequence of US31 was highly conserved while only a few strains had variation, and most of them were sense mutation. HCMV pUS31 contained 162 amino acid residues, the relative molecular mass was 20 kD and the isoelectric point was 7.66. It’s a soluble protein and its secondary structure occurred more frequently as random coil region. The predicted B-cell epitopes of the pUS31 might exist at N-terminal of amino acid sequence: 5~19, 32~47, 58~68, 110~124. Conclusion: The B-epitopes of pUS31 are predicted successfully, which provides a theoretic basis for the further study of protein characteristics, development of epitopes based vaccine, and preparation of monoclonal antibody against fusion protein.
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