急性单核细胞白血病特异性miRNA分子标志物的鉴定

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摘要目的：鉴定急性单核细胞白血病中特异高表达的microRNA（miRNA）。方法：通过分析急性单核细胞白血病细胞系（THP-1）与慢性粒细胞白血病细胞系（K562）的miRNA-seq数据，筛选一批在THP-1细胞系中显著高表达的miRNA，通过实时荧光定量PCR技术对这些miRNA在THP-1和K562细胞系中进行验证，获得在THP-1细胞系中特异高表达的miRNA，再通过实时荧光定量PCR技术在急性单核细胞白血病患者骨髓样品中进行验证，鉴定有望应用于该类疾病临床诊断的分子标志物。结果：最终筛选得到了3个在急性单核细胞白血病中特异高表达的miRNA分子标志物：let-7d-5p、miR-222-3p、miR-221-3p。结论：通过结合组学数据，利用实时荧光定量PCR技术在细胞系及临床样本中的验证，最终鉴定了3个在急性单核细胞白血病中特异高表达的miRNA分子标志物。

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	刘金立
	刘淑阁
	熊倩
	韩丽
	李伟
	王万恒
	张昭军
	李全贞

关键词 ：急性单核细胞白血病, miRNA, 实时荧光定量PCR, miRNA-Seq

Abstract：Objective: To characterize the molecular markers of microRNA (miRNA) with specifically high expression in acute monocytic leukemia. Methods: A batch of miRNAs was firstly screened out for their significantly higher expression in THP-1 (acute monocytic leukemia cell line) by analyzing high-throughput miRNA transcriptome sequencing data of THP-1 and K562 (chronic myeloid leukemia cell line). These miRNAs were further tested in the THP-1 and K562 cell line by quantitative real-time PCR (qRT-PCR) to obtain the specifically high expressed miRNAs in THP-1 cell line. Finally, these screened miNRAs were verified in bone marrow samples of acute monocytic leukemia patients by qRT-PCR. The finally verified miRNAs could serve the candidates for clinical diagnosis of this disease in the future. Results: Three miRNAs that are specifically highly expressed in acute monocytic leukemia including let-7d-5p, miR-221-3p and miR-222-3p were characteried. Conclusion: By integrating the bioinformatics analysis and verification in cell lines and patient bone marrow samples, we finally characterized 3 miRNAs as potential molecular diagnostic markers in acute monocytic leukemia.

Key words： acute monocytic leukemia microRNA quantitative real-time PCR miRNA-Seq

收稿日期: 2016-02-26

基金资助:国家重大科学仪器设备开发专项（2011YQ03013404）；国家高技术研究发展计划（863计划）（2015AA020108）。

通讯作者: 李全贞，教授，Email：Quan.Li@UTSouthwest-ern.edu；张昭军，教授，Email：Zhangzhaojun@big.ac.cn。

作者简介: 刘金立（1988-），女，山东德州人，硕士生。

链接本文:

https://xb.wmu.edu.cn/CN/ 或 https://xb.wmu.edu.cn/CN/Y2016/V46/I10/703

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