Effects of microRNA-212 on migration and invasion of gastric cancer cell lines SGC7901
YING Jianghui1, JIANG Peipei2, JIN Cancan2, PANG Wenyang2, CAI Yiqi2, ZHU Guanbao2.
1.Department of Burn Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Gastroenterological Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
YING Jianghui,JIANG Peipei,JIN Cancan, et al. Effects of microRNA-212 on migration and invasion of gastric cancer cell lines SGC7901[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(9): 644-648.
Abstract：Objective: To investigate the effect of human microRNA212 (miR-212) on the migration and invasion of gastric cancer cell line SGC7901. Methods: The i-miR-212 as the suppressor gene of miR-212 was transfected into SGC7901 cells according to different concentration (25, 50, 75, 100 nmol/L) via lipofectamin 2000. Empty vectors and unrelated fragment were also applied as controls. The expression of mature type miR-212 was detected by real-time PCR. Cell Counting Kit-8 was used to evaluate the effect of miR-212 on the proliferation of SGC-7901 cells, meanwhile Transwell assay for migration and invasion of SGC7901 cells. Results: Real-time PCR showed that cells Transfected with i-miR-212 had significantly low expression of miR-212, with the optimal concentration of miR-212 being 50 nmol/L; the expression of miR-212 was 18 times lower than empty group (P<0.01). The Cell Counting Kit-8 showed that the proliferation of cancer cells with i-miR-212 (50 nmol/L) was higher than that of the control group (P<0.01). The Transwells showed the migration and invasion of cancer cells with i-miR-212 (50 nmol/L) was higher than that of the control group (P<0.01), respectively. Conclusion: The i-miR-212 can effectively reduce miR-212 expression and promote the migration and invasion of SGC7901 cells. miR-212 may be a tumor suppressor gene in gastric cancer tumor cells.
