The role of caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro
WANG Ruili1, DAI Wei1, DAI Yuanrong2.
1.Department of Critical Care, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027; 2.Department of Respiratory Medicine, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325027
WANG Ruili,DAI Wei,DAI Yuanrong. The role of caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(9): 629-632.
Abstract：Objective: To explore the role of caveolae/caveolin-1 and P21 in the process of roxithromycin to inhibit the proliferation of asthmatic rats ASMCs in vitro. Methods: Recovery freeze-stored ASMCs of asthmatic rats were divided into five groups [group A (blank group), group R10 (roxithromycin 10 µg/mL), group R25 (roxithromycin 25 µg/mL), group R50 (roxithromycin 50 µg/mL) and group R100 (roxithromycin 100 µg/mL)]. The proliferation of ASMCs induced by roxithromycin were tested by CCK-8. The changes of caveolae in ASMCs were observed by transmission electron microscope. The protein expressions of caveolin-1 and P21 were measured by Western blot. Results: Roxithromycin could inhibit the proliferation of asthmatic rats ASMCs in vitro and had concentration-dependent manner. With the increased of intervention concentration, the expression of caveolae increased and the protein expressions of caveolin-1, P21 increased. Besides, compared with group A or group R10, the expressions of caveolin-1 and P21 protein in group R50 or R100 showed significantly different (P<0.05). There was significantly negative correlation between the cell activity rate of asthmatic rats and the expressions of caveolin-1 and P21 protein (r=－0.881, r=－0.951, P<0.05). The expression caveolin-1 was positively correlated with P21 (r=0.957, P<0.01). Conclusion: Roxithromycin inhibits the proliferation of ASMCs from asthmatic rats in vitro may partly through promoting the expression of P21 protein which induced by increased the expression of caveolae/ caveolin-1.
