CHENG Peng,HE Luqing,LING Xiao, et al. Increasing CXCR7 expression in endothelial progenitor cells can enhances its angiogenic capability[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(7): 507-512.
Abstract：Objective: To investigate whether increasing CXCR7 expression in endothelial progenitor cells (EPCs) can improve its angiogenic capability. Methods: CXCR7high-EPCs were constructed by transfecting with adenovirus carring human CXCR7 gene. The differences between CXCR7high-EPCs and wt-EPC in cell survival under serum deprivation condition, adhesion, SDF-1 induced trans-endothelial migration, tube formation and nitric oxide (NO) production were compared. Results: Compared with ctrl-EPCs, CXCR7high-EPCs has superiority in cell survival under serum-free condition, cell adhension to activated endothelial cells, trans-endothelial migration, tube formation and NO production, which could be further enhanced by SDF-1. Conclusion: Increasing expression of CXCR7 can enhance the angiogenic capacity of EPCs. The performance of present research may provide a new idea for the treatment of vascular diseases.
