ZHANG Fangfang,LIN Xiufei,CHEN Congcong, et al. Study on the role of Akt signaling in FGF21-induced prevention on diabetic carodiomyopathy[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(7): 490-496,502.
Abstract：Objective: To explore the role of fibroblast growth factor 21 (FGF21) on diabetic cardiomyopathy and analyze the role of Akt signaling in FGF21-induced prevention on diabetic carodiomyopathy. Methods: Male C57BL/6J mice were allowed to establish mouse model of type 2 diabetes. Diabetic mice were randomly selected for the treatment of FGF21 for 6 months. Heart tissues were obtained for detection of histopathological changes, cardiomyocyte hypertrophy, fibrosis index and apoptosis after heart functions were detected. Isolation and culture of primary myocardial cell in mice, high glucose/lipid (HG/Pal) treatment of cells to simulate the environment in type 2 diabetes mellitus, at the same time, FGF21 and Akt2-siRNA intervention were given for 39 hours to detect the change of cardiomyocyte hypertrophy, apoptosis and fibrosis. Results: It was demonstrated in vivo study that FGF21 could significantly alleviate heart structural changes, dysfunction, cardiomyocyte hypertrophy and fibrosis induced by type 2 diabetes (P<0.05). It was verified in vitro study demonstrated that FGF21 could upregulate Akt phosphorylation level, activation of Akt and downstream signaling pathways, inhibit the activity of Akt, abate FGF21 protection of myocardial injury. Conclusion: FGF21 plays a protective role in diabetes-induced myocardial injury, and Akt signaling is a key mechanism in FGF21-induced myocardial protection.
