Changes of the expression of signaling pathway of PI3K/Akt/GSK3β and endoplasmic reticulum stress in the formation of deep tissue injury of pressure ulcer in rats
1.Department of Nursing, Dongyang Hospital Affiliated to Wenzhou Medical University, Jinhua, 322100;
CUI Feifei1,ZHANG Jufang1,WU Haiying1, et al. Changes of the expression of signaling pathway of PI3K/Akt/GSK3β and endoplasmic reticulum stress in the formation of deep tissue injury of pressure ulcer in rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2014, 44(8): 555-560.
Abstract：Objective: To explore changes of the expression of signaling pathway of PI3K/Akt/GSK3β and expressions of ER chaperones in the deep tissue injury of pressure ulcer in rats. Methods: Fifty-four male Sprague-Dawley rats were divided into normal group and compressed groups (1 cycle, 3 cycles, 6 cycles, and 9 cycles group), natural recovery group as 1 day group, 3 days group, 5 days group and 7 days group according to the random number table, with six rats in each group. Muscle tissues underneath compression region and the same region normal group tissue were collected for analysis. Haematoxylin and eosin staining was carried out to examine the tissue histology; the expression of caspase-3 was assayed by immunohistochemical staining (Vectastain avidin-biotin complex, ABC); during different stages (experimental group and natual recovery group) the dynamic change of the ER chaperone such as GRP78, CHOP, caspase-12 and Akt, GSK3β, p-Akt, p-GSK3β in compressed muscle tissue were assayed by Western blot. Results: Under the light microscope of HE staining, the compressed muscle tissue of the experiment group showed a gradual degradation in the pathological changes, in recovery group, there were still signs of the pathlogical phenomenon; The immunohistochemical analysis indicated that the protein abundance of caspase-3 was elevated in the experiment group and natural recovery group compared with the control group, the detected immunoreactivity were generally distributed in cytoplasm. According to our immunoblot analysis, the protein expression of GRP78, CHOP, caspase-12 displayed a general ascending trend during different cycle; the protein expression of p-Akt showed first increased and then decreased; the protein expression of p-GSK3β offered first increased and then decreased with the time going, in recovery group p-GSK3β expression appeared first decreased and then increased with time going. Conclusion: The reasons of deep layer ulcers hard to heal may be related to muscle injury of the surrounding tissue; ERS and PI3K/Akt/GSK3β signaling pathway may involved in the procedure of deep ulcers.
