GAO Li,XIE Yangyang,WANG Xiang, et al. The role of Rho guanine nucleotide exchange factor 12 (ARHGEF12) in leukemia cell growth[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(6): 418-421.
Abstract：Objective: To assess the effect of ARHGEF12 on leukemia cell growth and the underlying mechanism. Methods: ARHGEF12 expression was determined by quantitative RT-PCR in several leukemia cell lines. Knock-down of LARG was performed with shRNAs in leukemia cell lines and the flow cytometry analysis (FACS) was used to detect the growth and apoptosis of the leukemia cells after LARG silencing. Antibody assay screen of kinase phosphorylation was done to explore the potential mechanism. Results: Knocking down ARHGEF12 in high expression cells would inhibit cell growth, which resulted from increased apoptosis and decreased proliferation. Phosphorylation of p53, CDK2 and p27 Kip1 were involved in this process. Conclusion: ARHGEF12 is essential for the growth of leukemia cells, with high expression of ARHGEF12, downregulation of ARHGEF12 would cause increased cell apoptosis and inhibited cell proliferation as well.
