Resveratrol ameliorates the lipid metabolism in the diet-induced obese zebrafish
RAN Gai1, YING Li1, LI Lin1, DENG Tuo1, YAN Qiaoqiao1, YING Chenjiang2, YE Xiaolei1.
1.School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou, 325035; 2.Department of Nutrition and Food Hygiene, School of Public Health, Huazhong University of Science and Technology, Wuhan, 430030
RAN Gai,YING Li,LI Lin, et al. Resveratrol ameliorates the lipid metabolism in the diet-induced obese zebrafish[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(6): 413-417.
Abstract：Objective: To investigate the effect of resveratrol (RSV) on lipid metabolism in diet-induced obese zebrafish. Methods: One hundred and twenty adult zebrafish (Danio rerio) of the wild-type strain (AB) were randomly allocated into 4 groups: control group (Con), Con+RSV, diet-induced obese (DIO), and DIO+RSV. The Con group was fed on freshly hatched live Artemia (8 mg cysts/fish/day) once a day for 8 weeks. The DIO group was fed on freshly hatched live Artemia (60 mg cysts/fish/day) three times a day for 8 weeks. The fish were exposed to 20 mmol/L resveratrol for 11 hours every day. After 8 weeks, the body length, body weight, total cholesterol (TC), and triglyceride (TG) were measured. The histology of the liver was evaluated. The protein levels of pAMPKα, AMPKα, Sirt1, caveolin-1 (Cav-1) and LC3-II were analyzed by western blotting. Results: After 8 weeks, the body length, body weight and condition factor increased by 11.7%, 61.9% and 15.1% (P<0.01), respectively. There was interaction effect on body length between the food intake and resveratrol (P=0.033), but resveratrol had no effect on the above parameters. Resveratrol could significantly alleviate the fatty infiltration and the formation of lipid droplets in the liver, and resveratrol up-regulated protein levels of pAMPKα, Sirt1 (725.8% DIO+RSV group vs. DIO group, P<0.01) and autophagy marker protein LC3-II (P=0.047). Conclusion: Without affecting the body length, body weight and lipid parameters, resveratrol ameliorates the lipid metabolism via pAMPK/Sirt1/autophagy pathway in the diet-induced obese zebrafish.
