Huang Tingting,GU Xuemei.. Effect of GLP-1 receptor agonist Exendin4 on secretion pattern of insulin and islet amyloid polypeptide in islet beta cells[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(6): 404-407,412.
Abstract：Objective: To explore the effect of GLP-1 receptor agonist (GLP-1RA) Exendin4 on insulin and islet amyloid polypeptide (IAPP) secretion pattern. Methods: Cell morphology of MIN6 cells was observed after treated with different concentrations of GLP-1 receptor agonist Exendin4 for different time periods. Cell viability of MIN6 cells was detected after different interventions respectively by MTT assay. Glucose-stimulated secretion levels of insulin and islet amyloid polypeptide were examined in culture supernatant of MIN6 cells and RT-PCR was used for detecting the expression levels of insulin mRNA and IAPP mRNA after different interventions respectively. Results: Compared with the control group, Exendin4 group exhibited much stronger adherence to the culture dish, with normal morphology and increasing cell numbers. There was a significant increase of cell viability in MIN6 cells treated with Exendin4, which displayed dose- and time-dependent pattern. Insulin and islet amyloid peptide secretion levels increased accordingly, while the ratio of IAPP/insulin decreased. The mRNA expression levels of insulin and IAPP of MIN6 cells were increased with treatment of Exendin4, which also displayed dose- and time-dependent pattern, while the ratio of IAPP/insulin mRNA correspondingly. Conclusion: Exendin4 increases the number and cell viability of MIN6 cells. Exposure to Exendin4 can increase insulin and IAPP secretion levels, and mRNA expression levels of insulin and IAPP as well, while the ratio of IAPP/insulin is deceased accordingly.
