Protective effect of curcumin against acute renal oxidative injury of paraquat-induced in mice
ZHENG Dan1, HAN Wenwen2, HONG Guangliang3, ZHAO Guangju3, LI Mengfang3, CAI Xiaoxia3, WU Bin3, LU Zhongqiu3
1.Intensive Care Unit, Taizhou Hospital Affiliated to Wenzhou Medical University, Taizhou, 317000; 2.Department of Emergency, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo, 315000; 3.Emergency Medical Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
ZHENG Dan,HAN Wenwen,HONG Guangliang, et al. Protective effect of curcumin against acute renal oxidative injury of paraquat-induced in mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(6): 391-396.
Abstract：Objective: To investigate the effect of curcumin on oxidative stress of acute renal injury in rats induced by paraquat and the mechanism underlying its effect. Methods: Sixty rats were randomly divided into 4 groups: control group (n=15), curcumin control group (curcumin 50 mg/kg) (n=15), PQ poisoned group (2% paraquat solution 100 mg/kg) (n=15), and curcumin intervention group (intraperitoneal injection of curcumin 50 mg/kg at 15 min, 24 h, or 48 h after paraquat exposure) (n=15). On days l, 3 and 7 after the treatment and renal tissue was collected. The content of malonaldehyde (MDA) and glutathione (GSH), the activities of superoxide dismutase activity (SOD) and catalase (CAT) in the renal tissue were determined with chemical colorimetry. The activities of heme oxygenase-1 (HO-1) and inducible nitric oxide synthase (iNOS) in the renal tissue were determined with ELISA. The mRNA and protein levels of nuclear related factor 2 (Nrf2) were determined with RT-PCR and Western Blot, respectively. The pathological changes of renal tissue were examined under optical microscopy. Results: Both PQ group and curcumin intervention group with each time showed increase in MDA content, decrease in GSH content, decrease in SOD and CAT activities, increase in iNOS activities, increase in the protein and mRNA levels of Nrf2, increase in HO-1 activities at day 1, 3, in comparison with the control group (P<0.05). In comparison with the PQ group on the same day, the curcumin intervention group showed decrease in MDA content, increase in the activities of GSH, SOD, CAT, decrease in iNOS activities, and increase in the mRNA and protein levels of Nrf2 on days l, 3 and 7, increase in HO-1 on days 1, 3 (P<0.05). In the PQ group, the clearance of glomerulus saccules was increased, arranged in disorder, renal tubular epithelial cell appeared glassy degeneration and vacuolar degeneration with time expanding, but compared with PQ group, the pathological manifestations was relieved obviously with the same time in the curcumin intervention group. Conclusion: Curcumin plays a protective role in paraquat poisoning with oxidative stress of acute renal injury, which may depend on inducing the expression of Nrf2, increasing the HO-1, SOD and other antioxidant enzyme levels, and increasing the ability of resistance to oxidative stress in renal tissue.
