QIU Weiwen,ZHENG Liyun,WU Zhiping, et al. The effects of naoxintong on vascular smooth muscle cells proliferation and autophagy induced by oxidized low-density lipoprotein[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(5): 340-343,347.
Abstract：Objective: To investigate the effects of naoxintong on the vascular smooth muscle cells (VSMC) proliferation and the expression of Beclin1 and LC3 induced by oxidized low-density lipoprotein (ox-LDL). Methods: The VSMC were divided into four groups: control group, ox-LDL group, naoxintong group and ox-LDL+naoxintong group. MTT and colony formation assay was used to test the proliferation. Western blot was used to analysis the expression of Beclin1 and LC3. Results: ox-LDL significantly promoted the viability and colony formation of VSMC. Naoxintong had no effect on the proliferation and colony formation but inhibited the proliferation and colony formation of VSMC induced by ox-LDL. Western blot results demonstrated that ox-LDL significantly promoted the expression of Becli1 and LC3, naoxintong inhibited the expression of Beclin1 and LC3 induced by ox-LDL. Furthermore, MTT verified that autophagy inhibitors 3MA inhibited significantly the proliferation induced by ox-LDL. Conclusion: Naoxintong inhibited the VSMC proliferation induced by ox-LDL through inhibited the cell autophagy.
