Screening and verification of circulating miRNA biomarkers of rheumatoid arthritis
PENG Wujian1, WANG Honglei2, HUANG Jianrong1, DAI Yong3.
1. Department of Nephrology, the Third People’s Hospital of Shenzhen, Shenzhen, 518020; 2.Department of Nephrology, the Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000; 3.Clinical Medical Research Center of the Second Clinical Medical College , Shenzhen People’s Hospital, Jinan University, Shenzhen, 518020
PENG Wujian,WANG Hongle,HUANG Jianrong, et al. Screening and verification of circulating miRNA biomarkers of rheumatoid arthritis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(5): 335-339.
Abstract：Objective: To analyze the differentl expression of circulating microRNAs (miRNAs) in patients with rheumatoid arthritis (RA). Methods: MiRNA profiles were performed to determine the differentially expressed miRNAs using RNA obtained from the plasma of patients with RA and healthy controls; to confirm array results, RT-qPCR was used for validation. GeneSpring GX software was to data processing, t-test and one way ANOVA were used for statistical analysis. Results: The array analysis identified 40 (18 upregulated miRNAs and 22 downregulated miRNAs) circulating miRNAs significantly differently expressed between RA and healthy controls, then we detected the candidate miRNAs selected based on fold-change and potential value by RT-qPCR, consistented with the data obtained from the array, the circulating level of candidate miRNAs were significantly differed in patients with RA compared with healthy controls (P<0.05). Conclusion: Differently expressed circulating miRNAs can be detected in patients with RA, this study indicates that circulating miRNAs may be used as a potential candidate biomarker of RA.
