Protective effect of ambroxol on experimental atelectasis
ZHANG Yili1, TU Junwei2, ZHOU Xiuyun3
1.Department of Critical-care Medicine, Jinhua Municipal Central Hospital, Jinhua, 321000; 2.Department of Respirology, Jinhua Municipal Central Hospital, Jinhua, 321000; 3.Department of Nephrology, Jinhua Municipal Central Hospital, Jinhua, 321000
ZHANG Yili,TU Junwei,ZHOU Xiuyun. Protective effect of ambroxol on experimental atelectasis[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(3): 187-189.
Abstract：Objective: To investigate the effect of ambroxol on experimental atelectasis. Methods: Eighteen male China experimental miniature pigs (CEMP) were randomly divided into three groups: contral group (C group), atelectasis group (M group) and ambroxol treatment group (T group), respectively (n=6). Ni-Ti alloys occluders were placed in the lower right main bronchus of M and T group animals with a bronchofiberscope after anesthesia. CEMP of T group were treated with high dose of ambroxol hydrochloride injection (30 mg/kg, q 8 h, respectively) after the occluders procedure was finished. The development of atelectasis was observed by computerized tomography scanning every three days after occluders were placed in. On the third day of atelectasis, bronchoalveolar lavage was carried out. The animals were killed at the end of experiment, dipalmitoylphosphatidylchol (DPPC), large aggregates (LA) and small aggregates (SA) from the bronchoalveolar lavage was measured using high performance liquid chromatography. SP-A from the bronchoalveolar lavage was measured using a sandwich ELISA technique. Histopathological changes of atelectasis were determined under a light microscope. Results: Typical pathology of atelectasis and computerized tomography imaging were observed in group M. Concentrations of DPPC, SP-A and rate of LA/SA in M group were significantly lower than that in C group, while concentrations of DPPC, SP-A and rate of LA/SA in T group were markly higer than those in M group. The area of atelectasis was shruken in T group compared to those in M group. Conclusion: These findings suggest that ambroxol has beneficial effect on experimental atelectasis. This may be the result that ambroxol can contribute to enhances pulmonary surfactant synthesis and reduce the conversion of LA to SA.
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