Establishment and identification of human cervical cancer SiHa cells tumor bearing model in nude mice
Hou Bailong1, Du Wangqi1, Xiong Yirong1, Cai Yiqi2, Song Yilin1, Ling XiaoYun1, Xue Xiangyang1, Zhang Lifang1
1.Institute of Molecular Virology and Immunolog, Department of Microbiology and Immunology, Wenzhou Medical University, Wenzhou, 325035; 2.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015
Hou Bailong,Du Wangqi,Xiong Yirong, et al. Establishment and identification of human cervical cancer SiHa cells tumor bearing model in nude mice[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(2): 92-96.
Abstract：Objective: To establish human cervical cancer SiHa cells tumor bearing model on the body surface area in nude mice, and analyze its tumor characteristics. Methods: Three groups nude mice were subcutaneous inoculated with 5×106/mL, 1×107/mL and 5×107/mL concentrations of cervical cancer SiHa cells in the right axillary close to upper limbs, to establish human cervical cancer SiHa cells tumor bearing model in nude mice. The tumor of nude mice was observed and the size of tumor was measured. The histological characteristics of tumor tissue was observed by pathological method after 5 weeks. HPV16E7 DNA of tumor tissue was comfirmed by PCR assay, and the expression of HPV16 E7 protein in tumor tissue was detected by immunohistochemistry method. Results: The tumor rate of 3 groups of nude mice inoculation with SiHa cell line was 100%. Their tumor sizes reached up (77.29±28.34) mm3, (178.91±61.55) mm3 and (305.11±12.62) mm3 after 5 weeks, and there was a significant difference among 3 groups (P<0.001). Pathology observation showed the growth of SiHa cell tumors were mainly in local infiltration. Histopathological of slice observation showed the new SiHa cells tumor tissue appeared pathological characteristics of large volume, large nucleus, nucleus deep dyeing and the mitotic phase. HPV16E7 DNA and the protein expression were positive comfirmed by PCR and immunohistochemistry detection respectively in tumor tissue. Conclusion: Human cervical cancer SiHa cells tumor bearing model in nude mice was established successfully, and provide an ideal animal model for human cervical cancer tumor targeted therapy and biological research.
