Effect of homocysteine on the phenotype transformation in the cultured rat vascular smooth muscle cell#br#
LI Gang1, MENG Liping1,2, ZHOU Changzuan2, JIANG Chengjian1, GUO Yan3, CHI Jufang1
1.Department of Cardiology, Shaoxing People’s Hospital, Shaoxing Hospital of Zhejiang University, Shaoxing,312000; 2.The First Clinical Medical College, Wenzhou Medical University, Wenzhou, 325035
LI Gang,MENG Liping,ZHOU Changzuan, et al. Effect of homocysteine on the phenotype transformation in the cultured rat vascular smooth muscle cell#br#[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(1): 33-38.
Abstract：Objective: To verify whether homocysteine (Hcy) could induce the dedifferentiation of vascular smooth muscle cells (VSMCs). Methods: The primary culture and identification of rat VSMCs was conducted using VSMCs in passage 4-7 for the following experiments. The VSMCs were divided into 4 groups: control group, Hcy (100 µmol/L) modulation group, Hcy (500 µmol/L) modulation group, Hcy (1 000 µmol/L) modulation group. MTT were used to investigate the proliferation of VSMCs. Transwell chambers and wound healing were employed to test the migratory ability of VSMCs. ICC were used to detect the VSMCs’ morphology structure. Western blotting was used to investigate the expressions of SM-actin, SM-MHC, Calponin, OPN in VSMCs of every group. Results: Compared with control group, the proliferation and migration ability of VSMCs were significantly increased in the Hcy modulation group (P<0.01). The expression of SM-actin had no significant difference between each group. The expression of SM-MCH and Calponin increased and OPN decreased in the Hcy group compared with control group (P<0.01). This effects of Hcy were positively correlated with its concentrations. Conclusion: Hcy can induce the dedifferentiation of VSMCs, this maybe the mechanism by which Hcy increases the proliferation and migration ability of VSMCs.
