Remodeling of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation
JIN Peifeng1, ZHANG Hao2, LU Di1, WENG Jiakan1, JIANG Sheng1, SUN Chengchao1.
1.Department of Thoracic and Cardiovascular Surgery, the First Affliliated Hospital of Wenzhou Medical University,Wenzhou, 325015; 2.Fu Wai Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing, 100037
JIN Peifeng,ZHANG Hao,LU Di, et al. Remodeling of parasympathetic/sympathetic ratio in the infarcted myocardium after Schwann cell transplantation[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2016, 46(1): 13-18.
Abstract：Objective: To investigated the feasibility of remodeling nervous system regeneration after myocardial infarction and the efficacy by which it may prevent ventricular arrhythmia following Schwann cells transplantation. Methods: Myocardial infarction was induced by left anterior descending artery ligation in syngenic Lewis rats, then the rats were randomized into cell transplantation group (n=31) and control group (n=30).Schwann cell were isolated from sciatic nerves, and immunohistochemical of S100 was carried out for the identification of Schwann cells. 5×106 Schwann cell were intramyocardially injected into the border zone of infarct region. The density of sympathetic and parasympathetic nerves and growth-associated protein 43 in the infarct region were stained by immunohistochemical method and immunofluorescence at 2 weeks after Schwann cells transplantation. Meanwhile, dynamic electrocardiography and programmed electric stimulation were also performed to detect the susceptibility of ventricular arrhythmias. Results: More than 95% of cells were S-100-positive Schwann cells during the entire culture process. Immunohistochemical and immunofluorescence staining illustrated increases in sympathetic and parasympathetic nerves in both groups. However, Schwann cells transplantation significantly increased the parasympathetic/sympathetic ratio at 2 weeks after cell injection. In addition,the Schwann cells also significantly decreased the low-/high-frequency ratio and susceptibility of programmed electric stimulation-induced ventricular arrhythmia at 2 weeks after cell injection. Conclusion: Transplanted Schwann cells in the infarcted myocardium can alter the ratio of parasympathetic/sympathetic nerve density to normalize irritable myocardium.
